Structure of PDB 8s9t Chain D

Receptor sequence

>8s9tD (length=506) Species: 1148 (Synechocystis sp. PCC 6803)

MARKVTTRWKITGTLIAETPLHIGGVGTDLALAVNGAGEYYVPGTSLAGA
LRGWMTQLLNNDESQIKDLWGDHLDAKRGASFVIVDDAVIHIPNNADVEI
REGVGIDRHFGTAANGFKYSRAVIPKGSKFKLPLTFDSQDDGLPNALIQL
LCALEAGDIRLGAAKTRGLGRIKLDDLKLKSFALDKPEGIFSALLDQGKK
LDWNQLKANVTYQSPPYLGISITWNPKDPVMVKAEGDGLAIDILPLVSQV
GSDVRFVIPGSSIKGILRTQAERIIRTICQSNGSEKNFLEQLRINLVNEL
FGSASLSDLGKIGALAVNDCFSSLSMTPDQWKAVENATEMTGNLQPALKQ
ATGYPNNISQAYKVLQPAMHVAVDRWTGGAAEGMLYSVLEPIGVTWEPIQ
VHLDIARLKNYYHGKEEKLKPAIALLLLVLRDLANKKIPVGYGTNRGMGT
ITVSQITLNGKALPTELEPLNKTMTCPNLTDLDEAFRQDLSTAWKEWIAD
PIDLCQ

3D structure

• PDB: 8s9t RNA targeting and cleavage by the type III-Dv CRISPR effector complex.
• Chain: D
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	G24 T49 S50 G53 A54 R56 G75 H77 G83 A84 S85 E106 G107 V108 I110 R112 T116 K122 Y123 G166 A167 A168 K169 T170 R171 K237 S265 S266 K268 G269 I270 R272 T273 F305 G306 S307 A308 S309 K323 I324 G325 M381 H382 V383 A384 V385 R387 G390 A392 L397 Y398 G453 Y454 G455 T456 N457 R458	G24 T45 S46 G49 A50 R52 G71 H73 G79 A80 S81 E102 G103 V104 I106 R108 T112 K118 Y119 G162 A163 A164 K165 T166 R167 K233 S261 S262 K264 G265 I266 R268 T269 F301 G302 S303 A304 S305 K311 I312 G313 M369 H370 V371 A372 V373 R375 G378 A380 L385 Y386 G441 Y442 G443 T444 N445 R446

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8s9t, PDBe:8s9t, PDBj:8s9t
• PDBsum: 8s9t
• PubMed: 38637512
• UniProt: Q6ZED3