Structure of PDB 8gri Chain D

Receptor sequence

>8griD (length=477) Species: 285500 (Streptomyces luteocolor)

VIVVGNGVLGLSVGVEIARTRPDVRVTLLGKPARQYGATPAAGAMLGAFG
EVTAHALASEHGRKKHALAVQAQRLWPEWIESLEATGTAADGRIKTADDT
VVLLNTVGHSALDDANFAAVLTALKEANAPHEEIAVESVDWIDPDPNSRP
LRALHIEGEGSVDSGILLAALERSFLQAGGRLHPVDATEIRASHGRVVVT
DDGDFLPAGHVVVAAGARSQRLVAALPGLAHRIPRIYDGVGVSALVDTWD
GSGPATVLRTSNRAFACGLHLVPRAGGSVYIGATNAVCLEPRGAASIAET
VFLFNCATHQLHRGLNGSELRKVQVGSRPAPIDGFPLIGGTSVEGLWMLS
GTYRDGLHMSPLLARHVVSLMDGGTGVDGLREFRPERDLISAWSREEILD
DVVRHTMATGYEFPWRLPLEWPHMMETFLQGPFAELADRLSDTYTPPADL
MTAIMFSEREQQDELIAYYADVHREWH

3D structure

• PDB: 8gri N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry.
• Chain: D
• Resolution: 2.365 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	I55	D	E434 M438	E420 M424
BS02	FAD	D	G17 N18 G19 L21 G42 R46 Q47 A50 T51 A54 M57 D198 A199 A229 G230 R232 G255 G340 R342 T366 Y367 R368 D369 G370 L371 H372	G5 N6 G7 L9 G30 R34 Q35 A38 T39 A42 M45 D186 A187 A215 G216 R218 G241 G326 R328 T352 Y353 R354 D355 G356 L357 H358
BS03	GLY	D	M57 H284 R342 R368	M45 H270 R328 R354
BS04	I55	D	N299 R306 S310 T423 T466 F470	N285 R292 S296 T409 T452 F456

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8gri, PDBe:8gri, PDBj:8gri
• PDBsum: 8gri
• PubMed: 37137912
• UniProt: A0A125SZC1