Structure of PDB 8e82 Chain D

Receptor sequence
>8e82D (length=1269) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
GAMLDVNFFDELRIGLATAEDIRQWSYGEVKKPETINYRTLKPEKDGLFC
EKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELA
APVTHIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITSVDEEMRHNEL
STLEAEMAVERKAVEDQRDGELEARAQKLEADLAELEAEGAKADARRKVR
DGGEREMRQIRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYRELVDRY
GEYFTGAMGAESIQKLIENFDIDAEAESLRDVIRNGKGQKKLRALKRLKV
VAAFQQSGNSPMGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVI
NRNNRLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRP
LKSLSDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMA
LELFKPFVMKRLVDLNHAQNIKSAKRMVERQRPQVWDVLEEVIAEHPVLL
NRAPTLHRLGIQAFEPMLVEGKAIQLHPLVCEAFNADFDGDQMAVHLPLS
AEAQAEARILMLSSNNILSPASGRPLAMPRLDMVTGLYYLTTEVPGDTGE
YQPASGDHPETGVYSSPAEAIMAADRGVLSVRAKIKVRLTQLRPPVEIEA
ELFGHSGWQPGDAWMAETTLGRVMFNELLPLGYPFVNKQMHKKVQAAIIN
DLAERYPMIVVAQTVDKLKDAGFYWATRSGVTVSMADVLVPPRKKEILDH
YEERADKVEKQFQRGALNHDERNEALVEIWKEATDEVGQALREHYPDDNP
IITIVDSGATGNFTQTRTLAGMKGLVTNPKGEFIPRPVKSSFREGLTVLE
YFINTHGARKGLADTALRTADSGYLTRRLVDVSQDVIVREHDCQTERGIV
VELAERAPDGTLIRDPYIETSAYARTLGTDAVDEAGNVIVERGQDLGDPE
IDALLAAGITQVKVRSVLTCATSTGVCATCYGRSMATGKLVDIGEAVGIV
AAQSIGEPGTQLTMRDITGGLPRVQELFEARVPRGKAPIADVTGRVRLED
GERFYKITIVPDDGGEEVVYDKISKRQRLRVFKRVLSDGDHVEVGQQLME
GSADPHEVLRVQGPREVQIHLVREVQEVYRAQGVSIHDKHIEVIVRQMLR
RVTIIDSGSTEFLPGSLIDRAEFEAENRRVVAEGGEPAAGRPVLMGITKA
SLATDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPAGTG
INRYRNIAVQPTEEARAAA
3D structure
PDB8e82 Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis.
ChainD
Resolution3.03 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D K288 K409 R414 R421 A501 A868 G871 Y872 Q1227 E1228 K290 K411 R416 R423 A503 A870 G873 Y874 Q1214 E1215
BS02 dna D R37 P122 K123 R1038 R39 P124 K125 R1031
BS03 rna D R397 D537 D539 R399 D539 D541
BS04 ZN D C60 C62 C78 C62 C64 C80
BS05 ZN D C891 C968 C975 C893 C970 C977
BS06 MG D D535 D537 D539 D537 D539 D541
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009274 peptidoglycan-based cell wall

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8e82, PDBe:8e82, PDBj:8e82
PDBsum8e82
PubMed37116494
UniProtP9WGY7|RPOC_MYCTU DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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