Structure of PDB 8cfw Chain D

Receptor sequence

>8cfwD (length=462) Species: 208964 (Pseudomonas aeruginosa PAO1)

AGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKIL
GCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFA
WKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLER
IHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSL
NDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICA
MQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDAN
MLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGF
DAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYA
DLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVS
VEGPFKPDTYRY

3D structure

• PDB: 8cfw Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry H05
• Chain: D
• Resolution: 1.61 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	D	K463 Y467	K456 Y460
BS02	RE4	D	D465 T466 R468	D458 T459 R461
BS03	NAD	D	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 G299 N300 I321 G322 H323 N375 H382	T158 T159 T160 N192 I220 G223 D224 V225 E244 V245 T291 G292 N293 I314 G315 H316 N368 H375
BS04	ADE	D	H61 T63 L376 H382 M387	H54 T56 L369 H375 M380

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfw, PDBe:8cfw, PDBj:8cfw
• PDBsum: 8cfw
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)