Structure of PDB 8b4h Chain D

Receptor sequence
>8b4hD (length=362) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]
MITRGEFFMIKEMYERGMSISDIARELGIDRKTVRKYIHSPNPPSKSKRK
QRKSKLDPFKPYLQKRMLEDGVFNSEKLFFEIRQQGYTGGKTILKDYMKP
FRETAKKKYTVRYETLPGEQMQVDWKEVGEVVIEGKKVKLSLFVATLGYS
RMKYAVFTTSQDQEHLMECLIQSFKYFGGVPKKVLFDNMKTVTDGREQGV
VKWNQRFSEFASYYGFIPKVCRRAIQYIMDHFYVGTAFESIEELNFLLHR
WLDQVANRKPNATTGISPQERWAEESLKPLPLKDYDTSYLSYRKVHWDGS
FSYKGEQWLLSAEYAGKEILVKERLNGDIRLYFRGEEISHVDQQKKVISF
AEKIKKKQTEMA
3D structure
PDB8b4h IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings.
ChainD
Resolution3.35 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 dna D I29 D30 K32 T33 Y37 R52 K53 N74 S75 R102 K139 D309 I29 D30 K32 T33 Y37 R52 K53 N74 S75 R102 K139 D298
BS02 dna D R31 K32 R35 R49 R52 S54 K55 G89 G90 T92 I93 R304 K305 H307 S313 Y314 K315 R31 K32 R35 R49 R52 S54 K55 G89 G90 T92 I93 R293 K294 H296 S302 Y303 K304
Gene Ontology
Molecular Function
GO:0000150 DNA strand exchange activity
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
Biological Process
GO:0006310 DNA recombination
GO:0015074 DNA integration
GO:0032196 transposition

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Molecular Function

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Biological Process
External links
PDB RCSB:8b4h, PDBe:8b4h, PDBj:8b4h
PDBsum8b4h
PubMed37087515
UniProtQ45618|TRA6_GEOSE Putative transposase for insertion sequence element IS5376

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