Structure of PDB 8a8u Chain D

Receptor sequence

>8a8uD (length=576) Species: 1773 (Mycobacterium tuberculosis)

SLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGE
PGVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEE
RLKKVLKEINTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGEL
QTIGATTLDEYRKYIEKDAALERRFQPVQVGEPTVEHTIEILKGLRDRYE
AHHRVSITDAAMVAAATLADRYINDRFLPDKAIDLIDEAGARMRIRRMVA
EVDDEQIAEVLGNWTGIPVFKLTEAETTRLLRMEEELHKRIIGQEDAVKA
VSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDAL
IQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEI
EKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTDYERMKQ
KVNDELKKHFRPEFLNRIDDIIVFHQLTREEIIRMVDLMISRVAGQLKSK
DMALVLTDAAKALLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVG
PGQVVTVDVDNWDGEGPGEDAVFTFT

3D structure

• PDB: 8a8u Structure of the drug target ClpC1 unfoldase in action provides insights on antibiotic mechanism of action.
• Chain: D
• Resolution: 3.62 Å

Enzymatic activity

• Enzyme Commision number: 3.4.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	D	R260 A297 F589 G600 V602	R93 A130 F362 G373 V375
BS02	ADP	D	P189 V190 I191 G219 G221 K222 T223 I358 L362	P22 V23 I24 G52 G54 K55 T56 I191 L195
BS03	ADP	D	I519 G556 V557 G558 K559 T560 E561 M730 A770 R771	I292 G329 V330 G331 K332 T333 E334 M485 A525 R526
BS04	ADP	D	R314 R340 R341	R147 R173 R174

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042803 protein homodimerization activity
• GO:0044183 protein folding chaperone

Biological Process

• GO:0006457 protein folding

Cellular Component

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8a8u, PDBe:8a8u, PDBj:8a8u
• PDBsum: 8a8u
• PubMed: 36208775
• UniProt: P9WPC9|CLPC1_MYCTU ATP-dependent Clp protease ATP-binding subunit ClpC1 (Gene Name=clpC1)