Structure of PDB 7xl4 Chain D

Receptor sequence

>7xl4D (length=1339) Species: 208964 (Pseudomonas aeruginosa PAO1)

EFDAIRIGLASPEMIRSWSFGEVKKPETINYRTFKPERDGLFCAKIFGPV
KDYECLCGKYKRLKHRGVICEKCGVEVALAKVRRERMGHIELASPVAHIW
FLKSLPSRIGLLLDMTLRDIERVLYFESYVVIDPGMTTLEKGQLLNDEQY
FEALEEFGDDFDARMGAEAVHELLNAIDLEHEIGRLREEIPQTNSETKIK
KLSKRLKLMEAFQGSGNKPEWMVLTVLPVLPPDLRPLVPLDGGRFATSDL
NDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRA
ITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPTLRLHQ
CGLPKKMALELFKPFIFGKLEGRGMATTIKAAKKMVERELPEVWDVLAEV
IREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQ
MAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVMGLYYMTR
EAINAKGEGMAFADLQEVDRAYRSGQASLHARVKVRINEKIKGEDGQLTA
NTRIVDTTVGRALLFQVVPAGLPFDVVNQSMKKKAISKLINHCYRVVGLK
DTVIFADQLMYTGFAYSTISGVSIGVNDFVIPDEKARIINAATDEVKEIE
SQYASGLVTQGEKYNKVIDLWSKANDEVSKAMMANLSKEKVVDREGKEVD
QESFNSMYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFRE
GLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEIDCG
TEHGLLMSPHIEGGDVVEPLGERVLGRVIARDVFKPGSDEVIVPAGTLID
EKWVDFLEVMSVDEVVVRSPITCETRHGICAMCYGRDLARGHRVNIGEAV
GVIAAQSIGEPGTQLTAADNVQVKNGGTIRLHNLKHVVRADGALVAVSRS
GELAVADDFGRERERYKLPYGAVISVKEGDKVDPGAIVAKWDPHTHPIVT
EVDGTVAFVGMEEGITVKRQTDELTGLTNIEVMDPKDRPAAGKDIRPAVK
LIDAAGKDLLLPGTDVPAQYFLPANALVNLTDGAKVSIGDVVARIPQTGG
LPRVADLFEARRPKEPSILAEISGTISFGKETKGKRRLVITPNDGSDPYE
ELIPKWRHLNVFEGEQVNRGEVISDGPSNPHDILRLLGVSSLAKYIVNEI
QDVYRLQGVKINDKHIETILRQMLRKVEVSESGDSSFIKGDQVELTQVLE
ENEQLGTEDKFPAKYERVLLGITKASLSTESFISAASFQETTRVLTEAAV
TGKRDFLRGLKENVVVGRLIPAGTGLAYHSERKRQRDLG

3D structure

• PDB: 7xl4 Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.
• Chain: D
• Resolution: 3.86 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	D	C85 C88	C70 C73
BS02	ZN	D	C888 C898	C873 C883
BS03	MG	D	D460 D462 D464	D445 D447 D449

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:7xl4, PDBe:7xl4, PDBj:7xl4
• PDBsum: 7xl4
• PubMed: 35859063
• UniProt: Q9HWC9|RPOC_PSEAE DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)