Structure of PDB 7utn Chain D

Receptor sequence

>7utnD (length=397) Species: 32630 (synthetic construct)

MAVVYVISKSGKPLMPTTRCGHVRILLKEGKARVVERKPFTIQLTYESAE
ETQPLVLGIDPGRTNIGMSVVTESGESVFNAQIETRNKDVPKLMKDRKQY
RMAHRRLKRRCKRRRRAKAAGTAFEEGEKQRLLPGCFKPITCKSIRNKEA
RFNNRKRPVGWLTPTANHLLVTHLNVVKKVQKILPVAKVVLELNRFSFMS
VLNQIIPYLADQLADMFPGNFCVTSGQDTYLFREEHGIPKDHYLDAYCIA
CSALTDAKKVSSPKGRPYMVHQFRRHDRQACHKANLNRSYYMGGKLVATN
RHKAMDQKTDSLEEYRAAHSAADVSKLTVKHPSAQYKDMSRIMPGSILVS
GEGKLFTLSRSEGRNKGQVNYFVSTEGIKYWARKCQYLRNNGGLQIY

3D structure

• PDB: 7utn Structural basis for RNA-guided DNA cleavage by IscB-omega RNA and mechanistic comparison with Cas9.
• Chain: D
• Resolution: 2.74 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	L14 M15 T17 R19 C20 R24 I25 K28 V34 V35 R37 F40 Y46 A49 E51 Q53 R86 M94 R97 Y100 R101 H104 R105 R106 R109 R110 K112 R113 R114 R115 R116 T122 F124 R131 P134 T141 K143 I145 R146 N147 K148 E149 A150 R151 F152 N153 N154 R155 K156 R157 P164 T165 H168 N175 F198 M199 R371 R372 H373 R375 Q376 A377 C378 R385 Y387 R398 H399 A401 M402 L409 R413 A418 S422 H428 M436 V446 Q483 L485 R486 N487	L14 M15 T17 R19 C20 R24 I25 K28 V34 V35 R37 F40 Y46 A49 E51 Q53 R86 M94 R97 Y100 R101 H104 R105 R106 R109 R110 K112 R113 R114 R115 R116 T122 F124 R131 P134 T141 K143 I145 R146 N147 K148 E149 A150 R151 F152 N153 N154 R155 K156 R157 P164 T165 H168 N175 F198 M199 R274 R275 H276 R278 Q279 A280 C281 R288 Y290 R301 H302 A304 M305 L312 R316 A321 S325 H331 M339 V349 Q386 L388 R389 N390
BS02	dna	D	C136 K138 F152 R157 G160 N300 Q301 Y305 K380 A381 N382 M402 Y468 W478	C136 K138 F152 R157 G160 N203 Q204 Y208 K283 A284 N285 M305 Y371 W381
BS03	dna	D	H379 Q432 R438 R457 S458 E459 G460 R461	H282 Q335 R341 R360 S361 E362 G363 R364

External links

• PDB: RCSB:7utn, PDBe:7utn, PDBj:7utn
• PDBsum: 7utn
• PubMed: 35617371