Structure of PDB 7uin Chain D

Receptor sequence

>7uinD (length=387) Species: 39491 (Agathobacter rectalis)

NLMEQILSSDNLNRAYLQVVRNKGAEGVDGMKYTELKEHLAKNGETIKGQ
LRTRKYKPQPGVPTVTDRFIQQAIAQVLTPIYEEQFHDHSYGFRPNRCAQ
QAILTALNIMNDGNDWIVDIDLEKFFDTVNHDKLMTLIGRTIKDGDVISI
VRKYLVSGGNLSPLLANIMLNELDKEMEKRGLNFVRYADDCIIMVGSEMS
ANRVMRNISRFIEEKLGLKVNMTKSKVDRPSGLKYLGFGFYFDPRAHQFK
AKPHAKSVAKFKKRMKELTCRSWGVSNSYKVEKLNQLIRGWINYFKIGSM
KTLCKELDSRIRYRLRMCIWKQWKTPQNQEKNLVKLGIDRNTARRVAYTG
KRIAYVCNKGAVNVAISNKRLASFGLISMLDYYIEKC

3D structure

• PDB: 7uin Structures of a mobile intron retroelement poised to attack its structured DNA target
• Chain: D
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	R58 D152 K153 T156 R160 I168 S169 R172 R217 S234 E235 M236 S237 R240 V241 R243 N244 R247 K303 R308 S309 R347 R351 M354 K358 R389 A391 N395	R54 D132 K133 T136 R140 I148 S149 R152 R180 S197 E198 M199 S200 R203 V204 R206 N207 R210 K266 R271 S272 R310 R314 M317 K321 R352 A354 N358
BS02	dna	D	R266 S268 Y278 F279 P281 R282 A283 F286 K289 S336 K338 T339 S346 R347 R349 Y350 K361 N378 R381 R382 V383 Y385 T386 R389 Y392 V393 K396 G397 A398 N400 S404 N405	R229 S231 Y241 F242 P244 R245 A246 F249 K252 S299 K301 T302 S309 R310 R312 Y313 K324 N341 R344 R345 V346 Y348 T349 R352 Y355 V356 K359 G360 A361 N363 S367 N368

Gene Ontology

Molecular Function

• GO:0003964 RNA-directed DNA polymerase activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process
• GO:0006974 DNA damage response

External links

• PDB: RCSB:7uin, PDBe:7uin, PDBj:7uin
• PDBsum: 7uin
• PubMed: 36356138
• UniProt: A0A173ZME3