Structure of PDB 7u22 Chain D

Receptor sequence

>7u22D (length=1265) Species: 1773 (Mycobacterium tuberculosis)

DVNFFDELRIGLATAEDIRQWSYGEVKKPETINYRTLKPEKDGLFCEKIF
GPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
HIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITSVDEEMRHNELSTLE
AEMAVERKAVEDQRDGELEARAQKLEADLAELEAEGAKADARRKVRDGGE
REMRQIRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYRELVDRYGEYF
TGAMGAESIQKLIENFDIDAEAESLRDVIRNGKGQKKLRALKRLKVVAAF
QQSGNSPMGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNN
RLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRPLKSL
SDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELF
KPFVMKRLVDLNHAQNIKSAKRMVERQRPQVWDVLEEVIAEHPVLLNRAP
TLHRLGIQAFEPMLVEGKAIQLHPLVCEAFNADFDGDQMAVHLPLSAEAQ
AEARILMLSSNNILSPASGRPLAMPRLDMVTGLYYLTTEVPGDTGEYQPA
SGDHPETGVYSSPAEAIMAADRGVLSVRAKIKVRLTQLRPPVEIEAELFG
HSGWQPGDAWMAETTLGRVMFNELLPLGYPFVNKQMHKKVQAAIINDLAE
RYPMIVVAQTVDKLKDAGFYWATRSGVTVSMADVLVPPRKKEILDHYEER
ADKVEKQFQRGALNHDERNEALVEIWKEATDEVGQALREHYPDDNPIITI
VDSGATGNFTQTRTLAGMKGLVTNPKGEFIPRPVKSSFREGLTVLEYFIN
THGARKGLADTALRTADSGYLTRRLVDVSQDVIVREHDCQTERGIVVELA
ERAPDGTLIRDPYIETSAYARTLGTDAVDEAGNVIVERGQDLGDPEIDAL
LAAGITQVKVRSVLTCATSTGVCATCYGRSMATGKLVDIGEAVGIVAAQS
IGEPGTQLTGGLPRVQELFEARVPRGKAPIADVTGRVRLEDGERFYKITI
VPDDGGEEVVYDKISKRQRLRVFKHEDGSERVLSDGDHVEVGQQLMEGSA
DPHEVLRVQGPREVQIHLVREVQEVYRAQGVSIHDKHIEVIVRQMLRRVT
IIDSGSTEFLPGSLIDRAEFEAENRRVVAEGGEPAAGRPVLMGITKASLA
TDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPAGTGINR
YRNIAVQPTEEARAA

3D structure

• PDB: 7u22 Redesign of Rifamycin Antibiotics to Overcome ADP-Ribosylation-Mediated Resistance.
• Chain: D
• Resolution: 3.87 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	R386 K409 R414	R384 K407 R412
BS02	dna	D	K294 R389 R1038 K1212	K292 R387 R1022 K1196
BS03	ZN	D	C891 C968 C975	C889 C966 C973
BS04	MG	D	D535 D537 D539	D533 D535 D537

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:7u22, PDBe:7u22, PDBj:7u22
• PDBsum: 7u22
• PubMed: 36222275
• UniProt: P9WGY7|RPOC_MYCTU DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)