Structure of PDB 7txu Chain D

Receptor sequence

>7txuD (length=869) Species: 1147 (Synechocystis sp. PCC 6714)

MKILKTLTLRGPNYWSIRRKKLIVMRLDLEDLAERPSNSIPGFYEGLIKV
LPSLVEHFCSPGYQGGFLERVKEGTYMGHIVEHVALELQELVGMTAGFGR
TRETSTPGVYNVVYEYVDEQAGRYAGRAAVRLCRSLVDTGDYPRLELEKD
LEDLRDLGANSALGPSTETIVTEAEARKIPWMLLSARAMVQLGYGVYQQR
IQATLSSHSGILGVELACDKEGTKTILQDAGIPVPRGTTIQYFDDLEEAI
NDVGGYPVVIKPLDGNHGRGITINVRHWQEAIAAYDLAAEESKIIVERYY
EGSDHRVLVVNGKLVAVAERIPAHVTGDGSSTISELIEKTNQDPNRGDGH
DNILTKIVVNKTAIDVMERQGYNLDSVLPKDEVVYLRATANLSTGGIAID
RTDDIHPENIWLMERVAKVIGLDIAGIDVVTSDISKPLRETNGVIVEVNA
APGFRMHVAPSQGLPRNVAAPVLDMLFPPGTPSRIPILAVTGTNGKTTTT
RLLAHIYRQTGKTVGYTSTDAIYINEYCVEKGDNTGPQSAGVILRDPTVE
VAVLETARGGILRAGLAFDSCDVGVVLNVAADHLGLGDIDTIEQMAKVKS
VIAEVVDPSGYAVLNADDPLVAAMADKVKAKVAYFSMNPDNPIIQAHVRR
NGIAAVYESGYLSILEGSWTLRVEQAKLIPMTMGGMAPFMIANALAACLA
AFVNGLDVEVIRQGVRTFTTSAEQTPGRMNLFNLGQHHALVDYAHNPAGY
RAVGDFVKNWQGQRFGVVGGPGDRRDSDLIELGQIAAQVFDRIIVKEDDD
KRGRSEGETADLIVKGILQENPGASYEVILDETIALNKALDQVEEKGLVV
VFPESVTRAIDLIKVRNPI

3D structure

• PDB: 7txu A cryptic third active site in cyanophycin synthetase creates primers for polymerization
• Chain: D
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.29: cyanophycin synthase (L-aspartate-adding).
  6.3.2.30: cyanophycin synthase (L-arginine-adding).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	D	G164 P165 S166 Q202 A203 A453	G164 P165 S166 Q202 A203 A450
BS02	peptide	D	Y14 S16 I17 R18 R19 H57 F58 L86 E87 E90 A96 G97 G99 R100 K600 E607	Y14 S16 I17 R18 R19 H57 F58 L86 E87 E90 A96 G97 G99 R100 K597 E604
BS03	MG	D	T500 T522 E558	T497 T519 E555
BS04	ZN	D	C59 H79 H83	C59 H79 H83
BS05	ATP	D	G268 I271 Y302 Y303 D307	G268 I271 Y299 Y300 D304
BS06	ATP	D	N497 G498 K499 T500 T522 F692 N696 D745 G752	N494 G495 K496 T497 T519 F689 N693 D742 G749

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity
• GO:0046872 metal ion binding
• GO:0071160 cyanophycin synthetase activity (L-aspartate-adding)
• GO:0071161 cyanophycin synthetase activity (L-arginine-adding)

Biological Process

• GO:0009058 biosynthetic process
• GO:0009059 macromolecule biosynthetic process

External links

• PDB: RCSB:7txu, PDBe:7txu, PDBj:7txu
• PDBsum: 7txu
• UniProt: A0A068N621