Structure of PDB 7tfj Chain D

Receptor sequence
>7tfjD (length=331) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
AQQPWVEKYRPKNLDEVTAQDHAVTVLKKTLKSANLPHMLFYGPPGTGKT
STILALTKELYGPDLMKSRILELNASDERGISIVREKVKNFARLTVSKPS
KHDLENYPCPPYKIIILDEADSMTADAQSALRRTMETYSGVTRFCLICNY
VTRIIDPLASRCSKFRFKALDASNAIDRLRFISEQENVKCDDGVLERILD
ISAGDLRRGITLLQSASKGAQYLGDGKNITSTQVEELAGVVPHDILIEIV
EKVKSGDFDEIKKYVNTFMKSGWSAASVVNQLHEYYITNDNFDTNFKNQI
SWLLFTTDSRLNNGTNEHIQLLNLLVKISQL
3D structure
PDB7tfj Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.
ChainD
Resolution3.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D I103 R107 I81 R85
BS02 AGS D V28 P33 V39 T40 P67 G68 T69 G70 K71 T72 S73 N171 R229 V6 P11 V17 T18 P45 G46 T47 G48 K49 T50 S51 N149 R207
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003689 DNA clamp loader activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000076 DNA replication checkpoint signaling
GO:0000077 DNA damage checkpoint signaling
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006272 leading strand elongation
GO:0006281 DNA repair
GO:0006298 mismatch repair
GO:0007062 sister chromatid cohesion
GO:0007064 mitotic sister chromatid cohesion
GO:0090618 DNA clamp unloading
Cellular Component
GO:0005634 nucleus
GO:0005663 DNA replication factor C complex
GO:0031389 Rad17 RFC-like complex
GO:0031390 Ctf18 RFC-like complex
GO:0031391 Elg1 RFC-like complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7tfj, PDBe:7tfj, PDBj:7tfj
PDBsum7tfj
PubMed35829698
UniProtP40348|RFC2_YEAST Replication factor C subunit 2 (Gene Name=RFC2)

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