Structure of PDB 7sxm Chain D

Receptor sequence

>7sxmD (length=547) Species: 79929 (Methanothermobacter marburgensis str. Marburg)

DKLFINALKKKFEESPEEKKTTFYTLGGWKQSERKTEFVNAGKEVAAKRG
IPQYNPDIGTPLGQRVLMPYQVSTTDTYVEGDDLHFVNNAAMQQMWDDIR
RTVIVGLNHAHAVIEKRLGKEVTPETITHYLETVNHAMPGAAVVQEHMVE
THPALVADSYVKVFTGNDEIADEIDPAFVIDINKQFPEDQAETLKAEVGD
GIWQVVRIPTIVSRTCDGATTSRWSAMQIGMSMISAYKQAAGEAATGDFA
YAAKHAEVIHMGTYLPVRRARGENEPGGVPFGYLADICQSSRVNYEDPVR
VSLDVVATGAMLYDQIWLGSYMSGGVGFTQYATAAYTDNILDDFTYFGKE
YVEDKYGLCEAPNNMDTVLDVATEVTFYGLEQYEEYPALLEDQFGGSQRA
AVVAAAAGCSTAFATGNAQTGLSGWYLSMYLHKEQHSRLGFYGYDLQDQC
GASNVFSIRGDEGLPLELRGPNYPNYAMNVGHQGEYAGISQAPHAARGDA
FVFNPLVKIAFADDNLVFDFTNVRGEFAKGALREFEPAGERALITPA

3D structure

• PDB: 7sxm XFEL serial crystallography reveals the room temperature structure of methyl-coenzyme M reductase.
• Chain: D
• Resolution: 2.503 Å

Enzymatic activity

• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F43	D	G326 V328 G329 F330 T331 Q332 Y333 G442 F443	G324 V326 G327 F328 T329 Q330 Y331 G440 F441
BS02	TP7	D	K256 H257	K254 H255
BS03	F43	D	A144 V145 V146 Q147 M150 M233 A243	A142 V143 V144 Q145 M148 M231 A241
BS04	TP7	D	R270 M324 F330 M480 N481	R268 M322 F328 M478 N479
BS05	COM	D	Y333 F443 Y444	Y331 F441 Y442

Gene Ontology

Molecular Function

• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7sxm, PDBe:7sxm, PDBj:7sxm
• PDBsum: 7sxm
• PubMed: 35202981
• UniProt: P11558|MCRA_METTM Methyl-coenzyme M reductase I subunit alpha (Gene Name=mcrA)