Structure of PDB 7on9 Chain D

Receptor sequence
>7on9D (length=390) Species: 649508 (Paenibacillus sp. JJ-1b) [Search protein sequence]
MRTQVGIIGAGPAGLLLSHLLYLQGIESIIIENRTREEIEGTIRAGVLEQ
GTVDLMNQMGVGARMMKEGHFHEGFELRFNGRGHRINVHELTGGKYVTVY
AQHEVIKDLVAARLQTGGQIHFNVGDVSLHDVDTSSPKIRFRPNKDGELQ
EIECDFIAGCDGFRGPSRPAIPQSVRKEYQKVYPFSWLGILVEAPPSAHE
LIYANHERGFALVSTRSPQIQRLYLQVDAQDHIDNWSDDRIWSELHARLE
TRDGFKLLEGPIFQKGIVSMRSFVCDPMQHGRLFLAGDAAHIVPPTGAKG
LNLAAADVQVLARGLEAYYKAGKMEILNRCTEICLRRIWKAERFSWFMTT
MLHRDQGHTPFERGIQLAELDYVTSSRAASTSLAENYIGL
3D structure
PDB7on9 Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate.
ChainD
Resolution1.63 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.14.13.33: 4-hydroxybenzoate 3-monooxygenase [NAD(P)H].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD D I8 G11 P12 A13 E32 N33 R34 R44 A45 V47 Q102 V127 D161 G287 D288 P295 A298 G300 L301 N302 I8 G11 P12 A13 E32 N33 R34 R44 A45 V47 Q102 V127 D161 G287 D288 P295 A298 G300 L301 N302
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0016491 oxidoreductase activity
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0018659 4-hydroxybenzoate 3-monooxygenase activity
GO:0018671 4-hydroxybenzoate 3-monooxygenase [NAD(P)H] activity
GO:0050660 flavin adenine dinucleotide binding
GO:0071949 FAD binding
GO:0106355 4-hydroxybenzoate 3-monooxygenase (NADH) activity
GO:0106356 4-hydroxybenzoate 3-monooxygenase (NADPH) activity
Biological Process
GO:0009056 catabolic process
GO:0043639 benzoate catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:7on9, PDBe:7on9, PDBj:7on9
PDBsum7on9
PubMed34982998
UniProtC4TP09|PRAI_PAESP 4-hydroxybenzoate 3-monooxygenase (NAD(P)H) (Gene Name=praI)

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