Structure of PDB 7nou Chain D

Receptor sequence

>7nouD (length=293) Species: 83332 (Mycobacterium tuberculosis H37Rv)

VIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNS
TRTRFSFELGIAQLGGHAVVVDSGGRDETLQDTAKVLSRYVDAIVWRTFG
QERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYF
GDGANNMAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGAS
VTVTADAHAAAAGADVLVTDTWTRVKPFRPFQLNSRLLALADSDAIVLHC
LPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALLVWLLERS

3D structure

• PDB: 7nou A fragment-based approach to assess the ligandability of ArgB, ArgC, ArgD and ArgF in the L-arginine biosynthetic pathway of Mycobacterium tuberculosis
• Chain: D
• Resolution: 1.98 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R101 H128 Q131 D224 C264 R292
• Catalytic site (residue number reindexed from 1): R97 H124 Q127 D220 C250 R278
• Enzyme Commision number: 2.1.3.3: ornithine carbamoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NMR	D	I45 D81 T87	I45 D77 T83
BS02	NMR	D	R52 F55 L265	R52 F55 L251
BS03	NMR	D	E82 V90 Y94	E78 V86 Y90

Gene Ontology

Molecular Function

• GO:0004585 ornithine carbamoyltransferase activity
• GO:0016597 amino acid binding
• GO:0016740 transferase activity
• GO:0016743 carboxyl- or carbamoyltransferase activity

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006526 L-arginine biosynthetic process
• GO:0006591 ornithine metabolic process
• GO:0019240 citrulline biosynthetic process
• GO:0042450 arginine biosynthetic process via ornithine

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7nou, PDBe:7nou, PDBj:7nou
• PDBsum: 7nou
• UniProt: P9WIT9|OTC_MYCTU Ornithine carbamoyltransferase (Gene Name=argF)