Structure of PDB 7mi9 Chain D

Receptor sequence
>7mi9D (length=339) Species: 243231 (Geobacter sulfurreducens PCA) [Search protein sequence]
RGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATLT
TAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPET
CLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAMR
APSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDPV
NALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPFR
PLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQE
VTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT
3D structure
PDB7mi9 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
ChainD
Resolution3.89 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D S287 Y288 W291 N411 R412 R413 P415 S424 F425 A428 H451 Q452 R454 R457 L462 N499 K506 S68 Y69 W72 N192 R193 R194 P196 S205 F206 A209 H232 Q233 R235 R238 L243 N280 K287
BS02 dna D K230 N265 T267 V375 F455 K11 N46 T48 V156 F236
BS03 dna D G381 F407 T408 G162 F188 T189
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:7mi9, PDBe:7mi9, PDBj:7mi9
PDBsum7mi9
PubMed34588691
UniProtQ74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)

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