Structure of PDB 7lo5 Chain D

Receptor sequence

>7lo5D (length=1012) Species: 980427 (Deinococcus wulumuqiensis) [Search protein sequence]

MSLQLVKKFQKRLEDIVAYGGTRNESSVRAAFQQLLSDWAEGSGLRLITE
VTQKAVAGNNVRPDGTLKDSLQQSRGYWESKDEADTLDDEIQKKLAKGYP
RDNIIFEDSRLAVLMQNGEEVQRVDMGDAGALAGLLKLFFEFEPPQVLEF
RKAVDHFKDEMPHLLKILREAADAAEQKADYRGERDHFVEIAKEAINPDF
SPRDAREMLIQHILTGDLFTSVFDNAQYHEDNNIAQQLQQLAATFYKGPV
KRDIAERTKRYYGAIQAAAAQIADHHEKQRFLKALYENFYRAYNPAGAER
LGIFYTPGEIVRFMIEATDTLLEKHFQKELADKGVEILDPATGTGTFITE
LIDFLPKAKLEQKYREELHCNELALLPYYIANLNIEATYAQKMGRYEEFR
NIVLVDTLDNTSGLFGSVTAENLERAKRQNARPVRVIIGNPPYRANQANE
NDNNKNREYKEIDRRIKATYVAASTAQKTKLYDMYSRFLRWATDRLKEDG
IVAFVSNSSFIDSRTFDGFRKEVVKDFDHIYILDMKGNANTSGERRKREG
GNVFNDQIKVGVAVYFLVRSAKDTKIWYHAVPDFWRAREKLEWLKTTKFE
DIEFDHIRPDAKHNWLGQVDEENDWNEFLPVADKDTKQAKGLGQERAIFK
LYSLGVVTNRDEWVYSRAEDELADKVRYFIGRYNEIIKLPLGDLMSRNWE
GDIKMTRATIADAQSRKSYSLEKNSIVPSLYRPFDVLKMYFSKNLNEMQY
QMPSIFPKGVGENVVIALSGSPAAKPFQVLATDILPSLDLLEKTQCLPFY
RYTMNGERLNNITDYALKAFQTHYAISREDIFHYVYAVLHHPAYREKYAL
NLRQEFPRIPFYPEFGRWAAWGRELMALHIGFESVAPYPLKRTDEPPKND
TPEALALAKKARLKVQRDAAKQPTGAVELDGLTTLAGIPAAAWAYKLGNR
SALEWVLERHKETTPKDATIREKFNTYRFADHKERVIDLLARVTTVSVET
VRIVGEMPAETM

3D structure

PDB

7lo5 Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.

Chain

Resolution

2.86 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	T52 N60	T52 N60
BS02	dna	D	N24 E25 S26 N59 V61 R62 K97 Y99	N24 E25 S26 N59 V61 R62 K97 Y99
BS03	dna	D	F304 N448 P449 P450 Y451 A453 Q485 K486 K488 N515 S521 R522 Q565 I566 K567 Y666 L668 M762 Y764 P786 L802 K807	F304 N440 P441 P442 Y443 A445 Q477 K478 K480 N507 S513 R514 Q557 I558 K559 Y652 L654 M748 Y750 P772 L788 K793
BS04	dna	D	N454 Q455 N457 E458 K463 K475 Q485 K486 Y490 A547 N548 S550 R554 R600 K651 N673 D675 K718 R721 Y745 R746 E761 D803 R967	N446 Q447 N449 E450 K455 K467 Q477 K478 Y482 A539 N540 S542 R546 R586 K637 N659 D661 K704 R707 Y731 R732 E747 D789 R950
BS05	CA	D	E25 D64 E79 S80	E25 D64 E79 S80
BS06	SAM	D	Y286 L301 G302 F304 Y305 T306 A341 T342 G343 T346 F347 E372 L373 D406 P450 M492 F496	Y286 L301 G302 F304 Y305 T306 A341 T342 G343 T346 F347 E372 L373 D406 P442 M484 F488

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0004519	endonuclease activity
GO:0008168	methyltransferase activity
GO:0008170	N-methyltransferase activity
GO:0009007	site-specific DNA-methyltransferase (adenine-specific) activity

	Biological Process
GO:0009307	DNA restriction-modification system
GO:0032259	methylation

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7lo5, PDBe:7lo5, PDBj:7lo5
PDBsum	7lo5
PubMed	33826880
UniProt	A0A345IJ72

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