Structure of PDB 7k98 Chain D

Receptor sequence

>7k98D (length=343) Species: 83332 (Mycobacterium tuberculosis H37Rv)

NAMLSPEALTTAVDAAQQAIALADTLDVLARVKTEHLGDRSPLALARQAL
AVLPKEQRAEAGKRVNAARNAAQRSYDERLATLRAERDAAVLVAEGIDVT
LPSTRVPAGARHPIIMLAEHVADTFIAMGWELAEGPEVETEQFNFDALNF
PADHPARGEQDTFYIAPEDSRQLLRTHTSPVQIRTLLARELPVYIISIGR
TFRTDELDATHTPIFHQVEGLAVDRGLSMAHLRGTLDAFARAEFGPSART
RIRPHFFPFTEPSAEVDVWFANKIGGAAWVEWGGCGMVHPNVLRATGIDP
DLYSGFAFGMGLERTLQFRNGIPDMRDMVEGDVRFSLPFGVGA

3D structure

• PDB: 7k98 Mycobacterium tuberculosis Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation.
• Chain: D
• Resolution: 2.19 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.20: phenylalanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	T32 R45 Q46 A49 R56 A57 G60 N64	T34 R47 Q48 A51 R58 A59 G62 N66
BS02	rna	D	F143 F148 H152 A154 Q158 D159 T174 R201 R324	F145 F150 H154 A156 Q160 D161 T176 R203 R326
BS03	W5Y	D	H175 R201 H209 T210 F213 Q215 E217 F255 F257 T258 W280 G282 C283 A305 F306 G307 G309 R312	H177 R203 H211 T212 F215 Q217 E219 F257 F259 T260 W282 G284 C285 A307 F308 G309 G311 R314

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000166 nucleotide binding
• GO:0000287 magnesium ion binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004826 phenylalanine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0006432 phenylalanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:7k98, PDBe:7k98, PDBj:7k98
• PDBsum: 7k98
• PubMed: 33885823
• UniProt: P9WFU3|SYFA_MYCTU Phenylalanine--tRNA ligase alpha subunit (Gene Name=pheS)