Structure of PDB 7fsc Chain D

Receptor sequence

>7fscD (length=425) Species: 9606 (Homo sapiens)

GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPV
AIALDTKGPGSGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRA
ALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKV
FLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGA
DCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSR
DPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRS
AQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVG
DLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fsc Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: D
• Resolution: 1.855 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	D	T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	T326 T327 T328 S331 W376 R383 G408 R410 P411 G412 S413 G414 Y415 T416
BS02	OXL	D	K282 E284 A305 G307 D308 T340	K164 E166 A187 G189 D190 T222
BS03	MG	D	E284 D308	E166 D190
BS04	ODL	D	F38 L39 Q405 L406 E409	F18 L19 Q287 L288 E291

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fsc, PDBe:7fsc, PDBj:7fsc
• PDBsum: 7fsc
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)