Structure of PDB 7fs2 Chain D

Receptor sequence

>7fs2D (length=425) Species: 9606 (Homo sapiens)

GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPV
AIALDTKGPGSGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRA
ALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKV
FLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGA
DCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSR
DPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRS
AQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVG
DLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fs2 Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: D
• Resolution: 2.374 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	O7X	D	F38 L365 A400 V401 Y402 Q405 L406	F18 L247 A282 V283 Y284 Q287 L288
BS02	FBP	D	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533	L325 T326 T327 T328 S331 W376 R383 G408 R410 P411 G412 S413 G414 Y415
BS03	OXL	D	K282 E284 A305 G307 D308	K164 E166 A187 G189 D190
BS04	MG	D	E284 D308	E166 D190

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fs2, PDBe:7fs2, PDBj:7fs2
• PDBsum: 7fs2
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)