Structure of PDB 7f0r Chain D

Receptor sequence
>7f0rD (length=1339) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence]
EFDAIRIGLASPEMIRSWSFGEVKKPETINYRTFKPERDGLFCAKIFGPV
KDYECLCGKYKRLKHRGVICEKCGVEVALAKVRRERMGHIELASPVAHIW
FLKSLPSRIGLLLDMTLRDIERVLYFESYVVIDPGMTTLEKGQLLNDEQY
FEALEEFGDDFDARMGAEAVHELLNAIDLEHEIGRLREEIPQTNSETKIK
KLSKRLKLMEAFQGSGNKPEWMVLTVLPVLPPDLRPLVPLDGGRFATSDL
NDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRA
ITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPTLRLHQ
CGLPKKMALELFKPFIFGKLEGRGMATTIKAAKKMVERELPEVWDVLAEV
IREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQ
MAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVMGLYYMTR
EAINAKGEGMAFADLQEVDRAYRSGQASLHARVKVRINEKIKGEDGQLTA
NTRIVDTTVGRALLFQVVPAGLPFDVVNQSMKKKAISKLINHCYRVVGLK
DTVIFADQLMYTGFAYSTISGVSIGVNDFVIPDEKARIINAATDEVKEIE
SQYASGLVTQGEKYNKVIDLWSKANDEVSKAMMANLSKEKVVDREGKEVD
QESFNSMYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFRE
GLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEIDCG
TEHGLLMSPHIEGGDVVEPLGERVLGRVIARDVFKPGSDEVIVPAGTLID
EKWVDFLEVMSVDEVVVRSPITCETRHGICAMCYGRDLARGHRVNIGEAV
GVIAAQSIGEPGTQLTAADNVQVKNGGTIRLHNLKHVVRADGALVAVSRS
GELAVADDFGRERERYKLPYGAVISVKEGDKVDPGAIVAKWDPHTHPIVT
EVDGTVAFVGMEEGITVKRQTDELTGLTNIEVMDPKDRPAAGKDIRPAVK
LIDAAGKDLLLPGTDVPAQYFLPANALVNLTDGAKVSIGDVVARIPQTGG
LPRVADLFEARRPKEPSILAEISGTISFGKETKGKRRLVITPNDGSDPYE
ELIPKWRHLNVFEGEQVNRGEVISDGPSNPHDILRLLGVSSLAKYIVNEI
QDVYRLQGVKINDKHIETILRQMLRKVEVSESGDSSFIKGDQVELTQVLE
ENEQLGTEDKFPAKYERVLLGITKASLSTESFISAASFQETTRVLTEAAV
TGKRDFLRGLKENVVVGRLIPAGTGLAYHSERKRQRDLG
3D structure
PDB7f0r Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.
ChainD
Resolution5.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 dna D T208 N209 S210 E211 E1327 T193 N194 S195 E196 E1290
BS02 dna D E1146 K1170 T1310 E1109 K1133 T1273
BS03 ZN D C70 C85 C55 C70
BS04 MG D D460 D462 D464 D445 D447 D449
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:7f0r, PDBe:7f0r, PDBj:7f0r
PDBsum7f0r
PubMed35859063
UniProtQ9HWC9|RPOC_PSEAE DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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