Structure of PDB 7cek Chain D

Receptor sequence

>7cekD (length=423) Species: 9823 (Sus scrofa)

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVY
YNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNN
WAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETY
CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLAVNMVPFPRLHFFMPGFAPLTSLTVPELTQQMFDSKNMMAACDPRHG
RYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPP
RGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEF
TEAESNMNDLVSEYQQYQDATAD

3D structure

• PDB: 7cek Small Molecules Promote Selective Denaturation and Degradation of Tubulin Heterodimers through a Low-Barrier Hydrogen Bond.
• Chain: D
• Resolution: 2.696 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	D	V175 Y222	V175 Y222
BS02	FW9	D	N165 F167 E198 Y200 V236 T237 C239 L240 L246 L250 L253 M257 A314 I376	N165 F167 E198 Y200 V236 T237 C239 L240 L246 L250 L253 M257 A306 I360
BS03	GTP	D	G10 Q11 C12 A97 N99 S138 G141 G142 T143 G144 E181 N204 Y222 N226	G10 Q11 C12 A97 N99 S138 G141 G142 T143 G144 E181 N204 Y222 N226

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005200 structural constituent of cytoskeleton
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000226 microtubule cytoskeleton organization
• GO:0000278 mitotic cell cycle
• GO:0007017 microtubule-based process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005874 microtubule

External links

• PDB: RCSB:7cek, PDBe:7cek, PDBj:7cek
• PDBsum: 7cek
• PubMed: 35762925
• UniProt: P02554|TBB_PIG Tubulin beta chain