Structure of PDB 7aud Chain D

Receptor sequence

>7audD (length=510) Species: 9606 (Homo sapiens)

HERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMP
TGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGD
KTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLA
RFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKD
ILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDS
GIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGC
QVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCL
LFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQ
LLAYFGENGFNPDFCKKHPDVSCDNCCSQREEMVKKCLGELTEVCKSLGK
VFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISV
LQKYSEWTSP

3D structure

• PDB: 7aud Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein.
• Chain: D
• Resolution: 2.96 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	L716 R717 T738 G739 T766 E768 K769 S773 R775 F807 R808 L896 S897 R898 H919 A920 T946 K968 R1003 L1004 E1008	L76 R77 T98 G99 T126 E128 K129 S133 R135 F167 R168 L256 S257 R258 H279 A280 T306 K328 R363 L364 E368
BS02	ZN	D	C1036 C1055 C1063	C396 C415 C423
BS03	ADP	D	L665 N667 Q672 T691 G692 G694 K695 S696	L25 N27 Q32 T51 G52 G54 K55 S56
BS04	RY8	D	S801 Q802 H805 D840 G972 Q975 T1015 T1018 N1022	S161 Q162 H165 D200 G332 Q335 T375 T378 N382

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006310 DNA recombination
• GO:0044237 cellular metabolic process

External links

• PDB: RCSB:7aud, PDBe:7aud, PDBj:7aud
• PDBsum: 7aud
• PubMed: 33647232
• UniProt: P54132|BLM_HUMAN RecQ-like DNA helicase BLM (Gene Name=BLM)