Structure of PDB 6wmu Chain D

Receptor sequence
>6wmuD (length=1363) Species: 562 (Escherichia coli) [Search protein sequence]
DLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPE
RDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMT
NLERQQILTEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLR
EELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPL
VPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEA
VDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGR
SVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVE
REEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPL
VCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVP
SQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRI
TEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAIS
KMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHE
IISEAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQ
TETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDG
SIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVD
VAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPG
TADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRD
LARGHIINKGEAIGVIAAQSIGEPGTQLTMAAAESSIQVKNKGSIKLSNV
KSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAG
GETVANWDPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLD
SAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGV
QISSGDTLARIPQESGGTKDITGGLPRVADLFEARRPKEPAILAEISGIV
SFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDG
PEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRK
ATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKAS
LATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGY
AYHQDRMRRRAAG
3D structure
PDB6wmu Structural Basis for Virulence Activation of Francisella tularensis.
ChainD
Resolution3.18 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D Y46 R47 Y44 R45
BS02 dna D R133 R1148 R131 R1135
BS03 dna D L120 K334 Y795 Q1326 E1327 L118 K332 Y793 Q1313 E1314
BS04 MG D D462 D464 D460 D462
BS05 ZN D C70 C72 C85 C68 C70 C83
BS06 ZN D C814 C888 C895 C898 C812 C886 C893 C896
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6wmu, PDBe:6wmu, PDBj:6wmu
PDBsum6wmu
PubMed33217319
UniProtP0A8T7|RPOC_ECOLI DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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