Structure of PDB 6wmt Chain D

Receptor sequence

>6wmtD (length=1159) Species: 376619 (Francisella tularensis subsp. holarctica LVS)

SKKFDIIKISLASPEVIRSWSHGEVKKPETINYRTFKPERDGLFCAKIFG
PIKDYECLCGKYKRLKHRGVVCERCGVEVEQAKVRRERMGHIDLVCPVVH
IWYLKSLPSRIGLFLDMPLKNVEKVLYFESYIVTDPGMTPLEKKQLLTDE
EYAEALENYGYEFEASMGAEAIRDLLADTDIESEIELLQAECEESKSTAK
KEKAIKRLRLLETFQASGNKPEWMVMTVLPVLPPDLRPLVPIEGGRFATS
DLNDLYRRVINRNNRLKKLLDLNAPDIIVRNEKRMLQEAVDALLDNGRRG
RAVTGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPSLRL
HECGLPKKMALELFKPFVYSKLRLGGHATTIKQAKRMVELEEAVVWDILE
TVINEHPVLLNRAPTLHRLGIQAFEPRLIEGKAIQLHPLVCAAFNADFDG
DQMAVHVPLTVESQLEARVLMMSTNNILSPASGQPIITPTQDIVLGLYYI
TREKEGARGEGKLFSSYEDVSRAYNSGTIDIHAKIKLRIDRQVFDTKGNT
YNEKGVVNTTVGRALLLNILPEGLSFSLLNKVLVKKEISKIINQAFRVLG
GKATVVLADKLMYAGFKYSTLSGVSVGVDDMTIPDNKEAKIEEAEKEIKQ
ITEQYQSSLITENERYNNIINIWSKTSDEVGASMMDAISKDTVSINGEKK
EIESFNSVYMMAKSGARGSYNQMRQLAGMRGLMAKPDGTMIETAITANFR
EGLSVLQYFTSTHGARKGLADTALKTANAGYLTRRLVDVAQDLVVIEEDC
GTDDGLMFSAIVEDGEVKVPLVERALGRTLAADVVTEKGVVLLEAGTLLD
ENLVELLDDNGIDMIKVRSPITCKTRRGLCAKCYGRDLARERQVNVGESV
GVIAAQSIGEPGTQLTMGLPRVAELFEARRPKDAAILSPCDGMVRLGNRD
TKEKQRIEIIDKNGHIVEEILLPKSRHLVVFDGEQVSRGDVLADGPTDPH
DLLKYKGLEEFADYILIEAQSVYRMQGVVINDKHIETIVRQMLRKAVILD
EGDSKFVKDESIELVRILEENDKLRKQGKKEVEYELVLMGITRSSLSTES
FLSAASFQETTRVLTEASINSQIDNLRGLKENVLIGRLIPAGTGLAVRKE
SAKIEKMRE

3D structure

• PDB: 6wmt Structural Basis for Virulence Activation of Francisella tularensis.
• Chain: D
• Resolution: 4.43 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	Y44 R45 R312	Y33 R34 R301
BS02	dna	D	T209 E1315	T198 E1109
BS03	MG	D	D460 D462	D449 D451
BS04	ZN	D	C68 C70 C83	C57 C59 C72
BS05	ZN	D	C811 C884 C891	C800 C873 C880
BS06	G4P	D	H362 K613 V616 D620	H351 K602 V605 D609

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0019865 immunoglobulin binding
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:6wmt, PDBe:6wmt, PDBj:6wmt
• PDBsum: 6wmt
• PubMed: 33217319
• UniProt: Q2A1M8|RPOC_FRATH DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)