Structure of PDB 6vwp Chain D

Receptor sequence

>6vwpD (length=435) Species: 83333 (Escherichia coli K-12)

HMKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLVDIEAK
VDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTM
HNYSVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPI
GRCFTLIGESGERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRC
KPGEPMPEATMKAIEYAKKYNVPVVLTLGTKFVIAENPQWWQQFLKDHVS
ILAMNEDEAEALTGESDPLLASDKALDWVDLVLCTAGPIGLYMAGFTEDE
AKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPLRVYSHIAPYMGGPEK
IMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYSSLAQVCK
YANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR

3D structure

• PDB: 6vwp ppGpp Coordinates Nucleotide and Amino-Acid Synthesis in E. coli During Starvation.
• Chain: D
• Resolution: 3.45 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.73: inosine kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	G4P	D	R7 S9 K10 H11 R302 F321 K383 S393 Q396	R8 S10 K11 H12 R303 F322 K384 S394 Q397
BS02	GMP	D	Q41 L43 D45 S65 G93 T94 C152 F164 Y195	Q42 L44 D46 S66 G94 T95 C153 F165 Y196
BS03	GMP	D	D276 V278 H327	D277 V279 H328

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008906 inosine kinase activity
• GO:0016301 kinase activity
• GO:0016772 transferase activity, transferring phosphorus-containing groups
• GO:0097216 guanosine tetraphosphate binding
• GO:0106366 guanosine kinase activity

Biological Process

• GO:0006166 purine ribonucleoside salvage
• GO:0016310 phosphorylation
• GO:0032263 GMP salvage
• GO:0032264 IMP salvage

External links

• PDB: RCSB:6vwp, PDBe:6vwp, PDBj:6vwp
• PDBsum: 6vwp
• PubMed: 32857952
• UniProt: P0AEW6|INGK_ECOLI Guanosine-inosine kinase (Gene Name=gsk)