Structure of PDB 6uqo Chain D

Receptor sequence

>6uqoD (length=578) Species: 83333 (Escherichia coli K-12)

MENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFK
ALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRV
IGSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAH
HDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRK
KTVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIE
ALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLR
FDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEK
AHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIG
LIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVE
LQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLAN
ELLFGSLVDGGQVTVALDKEKNELTYGF

3D structure

• PDB: 6uqo Conformational plasticity of the ClpAP AAA+ protease couples protein unfolding and proteolysis.
• Chain: D
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.4.21.92: endopeptidase Clp.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	D	K258 R260	K90 R92
BS02	peptide	D	G539 Y540 V541	G371 Y372 V373
BS03	AGS	D	L188 I189 S216 G217 G219 K220 T221 A222 I357 L361	L20 I21 S48 G49 G51 K52 T53 A54 I189 L193
BS04	AGS	D	V460 F461 T497 G498 V499 G500 K501 T502 E503 N606 F665 R702	V292 F293 T329 G330 V331 G332 K333 T334 E335 N438 F497 R534

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

External links

• PDB: RCSB:6uqo, PDBe:6uqo, PDBj:6uqo
• PDBsum: 6uqo
• PubMed: 32313240
• UniProt: P0ABH9|CLPA_ECOLI ATP-dependent Clp protease ATP-binding subunit ClpA (Gene Name=clpA)