Structure of PDB 6uca Chain D

Receptor sequence

>6ucaD (length=416) Species: 9606 (Homo sapiens)

MEVVLPLDPAVPAPLCPHGPTLLFVKVTQGAAATRRFYACSACRDRKDCN
FFQWEDEKLSGARLAAREAHNRRCQPPLSRTQCVERYLKFIELPLTQRKF
CQTCQQLLLPDDWGQHSEHQVLGNVSITQLRRPSQLLYPLENAATNAQYL
FADRSCQFLVDLLSALGFRRVLCVGTPRLHELIKLTASGDKKSNIKSLLL
DIDFRYSQFYMEDSFCHYNMFNHHFFDGKTALEVCRAFLQEDKGEGIIMV
TDPPFGGLVEPLAITFKKLIAMWKEGQSQDDSHKELPIFWIFPYFFESRI
CQFFPSFQMLDYQVDYDNHALYKHGKTGRKQSPVRIFTNIPPNKIILPTE
EGYRFCSPCQRYVSLENQHCELCNSCTSKDGRKWNHCFLCKKCVKPSWIH
CSICNHCAVPDHSCEG

3D structure

• PDB: 6uca Structure and regulation of ZCCHC4 in m6A-methylation of 28S rRNA.
• Chain: D
• Resolution: 3.103 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAH	D	N170 A171 Q172 Y173 L174 F175 G199 T200 P201 R202 D225 I226 Y242 N243 M244 F245 D276 P278	N146 A147 Q148 Y149 L150 F151 G175 T176 P177 R178 D201 I202 Y218 N219 M220 F221 D252 P254
BS02	ZN	D	H42 C73	H18 C49
BS03	ZN	D	C125 C128 H140 H143	C101 C104 H116 H119
BS04	ZN	D	C380 H393 C400	C356 H369 C376
BS05	ZN	D	C394 H410	C370 H386
BS06	ZN	D	C411 C414 H424 C431	C387 C390 H400 C407
BS07	ZN	D	C425 C428 H436 C438	C401 C404 H412 C414

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008988 rRNA (adenine-N6-)-methyltransferase activity

Biological Process

• GO:0031167 rRNA methylation
• GO:0032259 methylation

External links

• PDB: RCSB:6uca, PDBe:6uca, PDBj:6uca
• PDBsum: 6uca
• PubMed: 31695039
• UniProt: Q9H5U6|ZCHC4_HUMAN rRNA N6-adenosine-methyltransferase ZCCHC4 (Gene Name=ZCCHC4)