Structure of PDB 6t0f Chain D

Receptor sequence
>6t0fD (length=431) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
HHHMGLNTAIATRVNGTPPPEVPIADIELGSLDFWALDDDVRDGAFATLR
REAPISFWPTIELPGFVTGNGHWALTKYDDVFYASRHPDIFSSYPNITIN
DQTPELAEYFGSMIVLDDPRHQRLRSIVSRAFTPKVVARIEAAVRDRAHR
LVSSMIANNPDRQADLVSELAGPLPLQIICDMMGIPKADHQRIFHWTNVI
LGFGDPDLATDFDEFMQVSADIGAYATALAEDRRVNHHDDLTSSLVEAEV
DGERLSSREIASFFILLVVAGNETTRNAITHGVLALSRYPEQRDRWWSDF
DGLAPTAVEEIVRWASPVVYMRRTLTQDIELRGTKMAAGDKVSLWYCSAN
RDESKFADPWTFDLARNPNPHLGFGGGGAHFCLGANLARREIRVAFDELR
RQMPDVVATEEPARLLSQFIHGIKTLPVTWS
3D structure
PDB6t0f Metabolic Fate of Human Immunoactive Sterols in Mycobacterium tuberculosis.
ChainD
Resolution1.65 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G199 E270 T271 C379 L380 G381
Catalytic site (residue number reindexed from 1) G202 E273 T274 C382 L383 G384
Enzyme Commision number 1.14.15.14: methyl-branched lipid omega-hydroxylase.
1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM D I111 H118 R122 A267 G268 T271 T272 P314 R320 G370 F371 H377 C379 L380 G381 A385 I114 H121 R125 A270 G271 T274 T275 P317 R323 G373 F374 H380 C382 L383 G384 A388
BS02 K2B D F63 N93 T95 N97 L103 I197 L198 F212 F66 N96 T98 N100 L106 I200 L201 F215
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0031073 cholesterol 26-hydroxylase activity
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
GO:0070402 NADPH binding
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006707 cholesterol catabolic process
GO:0010430 fatty acid omega-oxidation
GO:0097089 methyl-branched fatty acid metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6t0f, PDBe:6t0f, PDBj:6t0f
PDBsum6t0f
PubMed33359098
UniProtP9WPP3|CP124_MYCTU Methyl-branched lipid omega-hydroxylase (Gene Name=cyp124)

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