Structure of PDB 6saq Chain D

Receptor sequence

>6saqD (length=417) Species: 224324 (Aquifex aeolicus VF5)

SYPAIPRIYAETTLNMLLKRAKKPRVHSIDEYLKDGGYQALEKALNMSPE
EIIDWVDKSTLRGRGGAGFPTGKKWKFAVQNPGPRYFICNADESEPGTFK
DRIIIERDPHLLIEGIIISSYAIGANEAYIYIRGEYPAGYYILRDAIEEA
KKKGFLGKNILGSGFDLEIYVARGAGAYICGEETALIESLEGKRGHPRLK
PPYPVQKGLWGKPTVVNNVETIANVPFIISMGWEEYRYIGPSDYAGPKLF
PVSGKVKKPGVYELPMNTTLREVIFKYAGGTLGNKKVKAVFSGALDCFSS
EELDIPMDYSPLGFGGTGTVIVLTEEDDIVEAALKIAEFYEHETCGQCTP
CRVGCYEQANLLEKIYKGEATEQDWEGFDFVNRNIQPTSICGLGAVAGRL
IRQTLEKFPEEWEKYRK

3D structure

• PDB: 6saq Structural Basis for Inhibition of ROS-Producing Respiratory Complex I by NADH-OH.
• Chain: D
• Resolution: 2.02 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	D	G65 G67 K76 N92 D94 E95 Y180 G183 E184 N219 N220 T223	G63 G65 K74 N90 D92 E93 Y178 G181 E182 N217 N218 T221
BS02	SF4	D	P199 T346 C347 G348 Q349 C350 C353 S391 C393 G396	P197 T344 C345 G346 Q347 C348 C351 S389 C391 G394
BS03	L3W	D	G67 G68 A69 F71 K76 F79 E95 E97 T100 E185 Y205 T319	G65 G66 A67 F69 K74 F77 E93 E95 T98 E183 Y203 T317

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:6saq, PDBe:6saq, PDBj:6saq
• PDBsum: 6saq
• PubMed: 34612584
• UniProt: O66841|NUOF_AQUAE NADH-quinone oxidoreductase subunit F (Gene Name=nuoF)