Structure of PDB 6p7e Chain D

Receptor sequence

>6p7eD (length=644) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSAIAANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQ
GEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTD
VGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARR
SELLRKLTETFGSWYAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKY
TDKGAPVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAW
LRYVAEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFG
AEHHLDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIH
TKNQIAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFL
ENTPAIAALRESIQQTLVESSQWQQVKWKRRWIKGLDGRKVHVRSPHAAL
NTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGC
RTEEIAQVVIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

6p7e Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome.

Chain

Resolution

3.001 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	D	R339 T357 V363 V364 D365 D366 K394 R429 A438 Q439 I440 P441 G442 H653	R283 T301 V307 V308 D309 D310 K338 R373 A382 Q383 I384 P385 G386 H593
BS02	dna	D	S338 G402 K404 V426 T431 H432 A433 G527 Y530 G531 A532 G533 R604 H607 N611 Q615	S282 G346 K348 V370 T375 H376 A377 G471 Y474 G475 A476 G477 R544 H547 N551 Q555
BS03	TTP	D	A7 A8 A10 Y64 W160	A7 A8 A10 Y64 W160
BS04	MG	D	D475 A476 D654	D419 A420 D594
BS05	MG	D	D475 D654	D419 D594
BS06	TTP	D	R429 D475 A476 G478 L479 E480 H506 R518 K522 Y526 D654	R373 D419 A420 G422 L423 E424 H450 R462 K466 Y470 D594

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:6p7e, PDBe:6p7e, PDBj:6p7e
PDBsum	6p7e
PubMed	31659895
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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