Structure of PDB 6oax Chain D

Receptor sequence

>6oaxD (length=581) Species: 83333 (Escherichia coli K-12)

LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGV
GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLK
GVLNDLAKQEGNVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHC
VGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYELH
HHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKRL
LRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEA
VDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSD
EAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILL
DEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQ
ERFGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQ
IQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIE
NPLAQQILSGELVPGKVIRLEVNEDRIVAVQ

3D structure

• PDB: 6oax Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
• Chain: D
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	D	K250 Y251 D290 G652 Y653 V654	K90 Y91 D130 G376 Y377 V378
BS02	AGS	D	R331 R332	R171 R172
BS03	AGS	D	V180 I181 G209 G211 K212 T213 A214 I349 I391	V20 I21 G49 G51 K52 T53 A54 I189 I231
BS04	AGS	D	I571 T607 G608 V609 G610 K611 T612 E613 E678 I774 Q778 R815 K818	I295 T331 G332 V333 G334 K335 T336 E337 E402 I498 Q502 R539 K542

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009408 response to heat
• GO:0034605 cellular response to heat
• GO:0042026 protein refolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:6oax, PDBe:6oax, PDBj:6oax
• PDBsum: 6oax
• PubMed: 31160557
• UniProt: P63284|CLPB_ECOLI Chaperone protein ClpB (Gene Name=clpB)