Structure of PDB 6klb Chain D

Receptor sequence

>6klbD (length=1115) Species: 1898203 (Lachnospiraceae bacterium)

MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGV
KKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEIN
LRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTA
FTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKH
EVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGE
KIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSGYTSDEEVLEVFRNTLNK
NSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRD
KWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSV
VEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLL
DSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNY
VTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDK
KYAKCLQKIDKDDVNGNYEKINYKVSFESASKKEVDKLVEEGKLYMFQIY
NKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEE
LVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKC
PKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYS
LNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYIS
QVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLN
YMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKID
PSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRT
DADYIKKWKLYSYGNRIRIFRFDWEEVCLTSAYKELFNKYGINYQQGDIR
ALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFY
DSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIA
ISNKEWLEYAQTSVK

3D structure

• PDB: 6klb Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.
• Chain: D
• Resolution: 4.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	S14 K15 T16 R18 K20 Y516 K518 N706 K707 S713 T716 N718 L719 H720 R747 H759 I765 N767 K768 K774 T777 L779 Y781 K785 D786 K787 R788 E795 F863 Y872 L875 E898 Q906 H909 K953 K960	S14 K15 T16 R18 K20 Y506 K508 N601 K602 S608 T611 N613 L614 H615 R642 H654 I660 N662 K663 K669 T672 L674 Y676 K680 D681 K682 R683 E690 F758 Y767 L770 E793 Q801 H804 K848 K855

External links

• PDB: RCSB:6klb, PDBe:6klb, PDBj:6klb
• PDBsum: 6klb
• PubMed: 31467167