Structure of PDB 6cip Chain D

Receptor sequence

>6cipD (length=1164) Species: 264732 (Moorella thermoacetica ATCC 39073)

PKQTLDGNTAAAHVAYAMSEVATIYPITPSSPMAEIADEWAAHGRKNIFG
KTLQVAEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAGE
LLPCVFHVAARALSTHALSIFGDHADVMAARQTGFAMLSSASVQEVMDLA
LVAHLATLKARVPFVHFFDGFRTSHEVQKIDVIEYEDMAKLVDWDAIRAF
RQRALNPEHPHQRGTAQNPDIYFQSREAANPYYLATPGIVAQVMEQVAGL
TGRHYHLFDYAGAPDAERVIVSMGSSCEVIEETVNYLVEKGEKVGLIKVR
LFRPFSAEHFLKVLPASVKRIAVLDRTKEPGSLGEPLYEDVQTVLAEHGK
NILVVGGRYGLGSKEFNPSMVKAVFDNLAATTPKNKFTVGITDDVTHTSL
EIKEHIDTSPKGTFRCKFFGLGSDGTVGANKNSIKIIGDHTDMYAQGYFV
YDSKKSGGVTISHLRFGKQPIQSAYLIDQADLIACHNPSYVGRYNLLEGI
KPGGIFLLNSTWSAEEMDSRLPADMKRTIATKKLKFYNIDAVKIAQEIGL
GSRINVIMQTAFFKIANVIPVDEAIKYIKDSIVKTYGKKGDKILNMNFAA
VDRALEALEEIKYPASWADAVDETEEPEFIQKVLRPINALKGDELPVSTF
TPDGVFPVGTTKYEKRGIAVNIPQWQPENCIQCNQCSLVCPHAAIRPYLA
KPADLAGAPETFVTKDAIGKEAAGLKFRIQVSPLDCTGCGNCADVCPAKV
KALTMVPLEEVTAVEEANYNFAEQLPEVKVNFNPATVKGSQFRQPLLEFS
GACAGCGETPYVKLVTQLFGDRMIIANATGCSSIWGGSAPACPYTVNRQG
HGPAWASSLFEDNAEFGYGMALAVAKRQDELATAISKALEAPVSAAFKAA
CEGWLAGKDDADRSREYGDRIKALLPGEISQASGEVKDLLLDIDRQKDYL
TKKSIWIIGGDGWAYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQSS
KATQTGAVARFAAGGKFTKKKDLGLMAMSYGYVYVASVAMGASHSQLMKA
LIEAEKYDGPSLIIAYAPCINHGINMTYSQREAKKAVEAGYWPLYRYNPQ
LAQEGKNPFILDYKTPTASFRDFLMGEIRYTSLKKQFPEKAEQLFAKAEA
DAKARLEQYKKLAE

3D structure

• PDB: 6cip Binding site for coenzyme A revealed in the structure of pyruvate:ferredoxin oxidoreductase fromMoorella thermoacetica.
• Chain: D
• Resolution: 3.189 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T29 E62 R112 N1000
• Catalytic site (residue number reindexed from 1): T28 E61 R111 N994
• Enzyme Commision number: 1.2.7.1: pyruvate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	D	W681 C686 I687 C689 N690 C692 I735 C752	W675 C680 I681 C683 N684 C686 I729 C746
BS02	SF4	D	P679 C696 I701 C742 T743 G744 C745 G746 C748	P673 C690 I695 C736 T737 G738 C739 G740 C742
BS03	SF4	D	A808 C809 C812 E814 C837 C1075	A802 C803 C806 E808 C831 C1069
BS04	HTL	D	P27 I28 E62 Q86 R112 E814 T835 G836 C837 F866 G966 D967 G968 V997 Y998 S999 N1000 T1001	P26 I27 E61 Q85 R111 E808 T829 G830 C831 F860 G960 D961 G962 V991 Y992 S993 N994 T995
BS05	MG	D	D967 T995 V997	D961 T989 V991

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005506 iron ion binding
• GO:0016491 oxidoreductase activity
• GO:0016903 oxidoreductase activity, acting on the aldehyde or oxo group of donors
• GO:0019164 pyruvate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006086 acetyl-CoA biosynthetic process from pyruvate
• GO:0006979 response to oxidative stress
• GO:0022900 electron transport chain

External links

• PDB: RCSB:6cip, PDBe:6cip, PDBj:6cip
• PDBsum: 6cip
• PubMed: 29581263
• UniProt: Q2RMD6|PFOR_MOOTA Pyruvate:ferredoxin oxidoreductase (Gene Name=Moth_0064)