Structure of PDB 5x8g Chain D

Receptor sequence
>5x8gD (length=482) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168) [Search protein sequence]
TEQPNWLMQRAQLTPERIALIYEDQTVTFAELFAASKRMAEQLAAHSVRK
GDTAAILLQNRAEMVYAVHACFLLGVKAVLLNTKLSTHERLFQLEDSGSG
FLLTDSSFEKKEYEHIVQTIDVDELMKEAAEEIEIEAYMQMDATATLMYT
SGTTGKPKGVQQTFGNHYFSAVSSALNLGITEQDRWLIALPLFHISGLSA
LFKSVIYGMTVVLHQRFSVSDVLHSINRHEVTMISAVQTMLASLLEETNR
CPESIRCILLGGGPAPLPLLEECREKGFPVFQSYGMTETCSQIVTLSPEF
SMEKLGSAGKPLFSCEIKIERDGQVCEPYEHGEIMVKGPNVMKSYFNRES
ANEASFQNGWLKTGDLGYLDNEGFLYVLDRRSDLIISGGENIYPAEVESV
LLSHPAVAEAGVSGAEDKKWGKVPHAYLVLHKPVSAGELTDYCKERLAKY
KRPKKFFVLDRLPRNASNKLLRNQLKDRKGEL
3D structure
PDB5x8g Crystal structure of the thioesterification conformation of Bacillus subtilis o-succinylbenzoyl-CoA synthetase reveals a distinct substrate-binding mode
ChainD
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T152 S172 H196 T289 E290 I388 N393 K471
Catalytic site (residue number reindexed from 1) T150 S170 H194 T287 E288 I386 N391 K469
Enzyme Commision number 6.2.1.26: o-succinylbenzoate--CoA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 S0N D K86 P193 H196 I197 S198 F219 A238 V239 L261 G263 G287 S293 Q294 S389 G390 G391 E392 K421 W422 K451 Y452 K84 P191 H194 I195 S196 F217 A236 V237 L259 G261 G285 S291 Q292 S387 G388 G389 E390 K419 W420 K449 Y450
BS02 MG D K320 Q359 W362 K318 Q357 W360
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008756 o-succinylbenzoate-CoA ligase activity
GO:0016405 CoA-ligase activity
GO:0016874 ligase activity
Biological Process
GO:0009234 menaquinone biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:5x8g, PDBe:5x8g, PDBj:5x8g
PDBsum5x8g
PubMed28559280
UniProtP23971|MENE_BACSU 2-succinylbenzoate--CoA ligase (Gene Name=menE)

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