Structure of PDB 5uhf Chain D

Receptor sequence

>5uhfD (length=1265) Species: 83332 (Mycobacterium tuberculosis H37Rv)

DVNFFDELRIGLATAEDIRQWSYGEVKKPETINYRTLKPEKDGLFCEKIF
GPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
HIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITSVDEEMRHNELSTLE
AEMAVERKAVEDQRDGELEARAQKLEADLAELEAEGAKADARRKVRDGGE
REMRQIRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYRELVDRYGEYF
TGAMGAESIQKLIENFDIDAEAESLRDVIRNGKGQKKLRALKRLKVVAAF
QQSGNSPMGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNN
RLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRPLKSL
SDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELF
KPFVMKRLVDLNHAQNIKSAKRMVERQRPQVWDVLEEVIAEHPVLLNRAP
TLHRLGIQAFEPMLVEGKAIQLHPLVCEAFNADFDGDQMAVHLPLSAEAQ
AEARILMLSSNNILSPASGRPLAMPRLDMVTGLYYLTTEVPGDTGEYQPA
SGDHPETGVYSSPAEAIMAADRGVLSVRAKIKVRLTQLRPPVEIEAELFG
HSGWQPGDAWMAETTLGRVMFNELLPLGYPFVNKQMHKKVQAAIINDLAE
RYPMIVVAQTVDKLKDAGFYWATRSGVTVSMADVLVPPRKKEILDHYEER
ADKVEKQFQRGALNHDERNEALVEIWKEATDEVGQALREHYPDDNPIITI
VDSGATGNFTQTRTLAGMKGLVTNPKGEFIPRPVKSSFREGLTVLEYFIN
THGARKGLADTALRTADSGYLTRRLVDVSQDVIVREHDCQTERGIVVELA
ERAPDGTLIRDPYIETSAYARTLGTDAVDEAGNVIVERGQDLGDPEIDAL
LAAGITQVKVRSVLTCATSTGVCATCYGRSMATGKLVDIGEAVGIVAAQS
IGEPGTQLTGGLPRVQELFEARVPRGKAPIADVTGRVRLEDGERFYKITI
VPDDGGEEVVYDKISKRQRLRVFKHEDGSERVLSDGDHVEVGQQLMEGSA
DPHEVLRVQGPREVQIHLVREVQEVYRAQGVSIHDKHIEVIVRQMLRRVT
IIDSGSTEFLPGSLIDRAEFEAENRRVVAEGGEPAAGRPVLMGITKASLA
TDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPAGTGINR
YRNIAVQPTEEARAA

3D structure

• PDB: 5uhf Structural Basis of Mycobacterium tuberculosis Transcription and Transcription Inhibition.
• Chain: D
• Resolution: 4.345 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	K409 R414 A868	K407 R412 A866
BS02	ZN	D	C891 C968 C975 C978	C889 C966 C973 C976
BS03	ZN	D	C62 C75	C60 C73
BS04	MG	D	D535 D537 D539	D533 D535 D537
BS05	88D	D	R834 L847 E848 F850 I851	R832 L845 E846 F848 I849

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:5uhf, PDBe:5uhf, PDBj:5uhf
• PDBsum: 5uhf
• PubMed: 28392175
• UniProt: P9WGY7|RPOC_MYCTU DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)