Structure of PDB 5twj Chain D

Receptor sequence
>5twjD (length=157) Species: 562 (Escherichia coli) [Search protein sequence]
HHVKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRGKNAD
IKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDV
SLLIGGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITT
NHPYHRE
3D structure
PDB5twj Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine.
ChainD
Resolution2.299 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.177: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAM D L72 D73 I74 G103 G107 L122 L127 V132 L74 D75 I76 G105 G109 L124 L129 V134 MOAD: Kd=5.58uM
PDBbind-CN: -logKd/Ki=5.25,Kd=5.58uM
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0042803 protein homodimerization activity
GO:0043022 ribosome binding
GO:0070038 rRNA (pseudouridine-N3-)-methyltransferase activity
Biological Process
GO:0006364 rRNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0070475 rRNA base methylation
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:5twj, PDBe:5twj, PDBj:5twj
PDBsum5twj
PubMed28428565
UniProtP0A8I8|RLMH_ECOLI Ribosomal RNA large subunit methyltransferase H (Gene Name=rlmH)

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