Structure of PDB 5nsm Chain D

Receptor sequence

>5nsmD (length=519) Species: 185431 (Trypanosoma brucei brucei TREU927)

TLPKAEAKELSAFVQSCVEYKTNVCFTDVAAYESNQKGVLSSGLAVLVGT
HKQLRDPAVQRLPFYNPAVAEAIERVKEGGTYGVLVEGLANAAGSKFVRV
VVGEVPTKASRNNCPARPDVVTALVTAALDEVKEPNTTVDVFVLSNAVLP
IAAAVARCGKHNFSAKDGAAAAAYNSGKVSRLQVVFPEPPAIPPKDLEAV
ATSTQLCQRLVDAPPNLLTTATFTEIAQGYAKALGFDVDVICGDDLCERG
YGGIYSVGKAAFEAPRLVTLLYTPKGTPVKKVSLVGKGIVYDCGGLALKP
ADYMKLMKHDMGGAAAVFCGFLTAVRLQQPVQLSCTLCLAENAIGPKSYR
NDDIIVMKSGKTVEVINTDAEGRIVLGDGVFHATNELSFTPDVVIDMATL
TGAQGIATGRHHAGLYVNEEGAEAAMLRAGRESGETCFPVLYCPEYHEPE
FKSNHADMTNLMERRDNAGVSCAGYFITTHLSPKFTGAHIHVDLAYPVFN
SNGATGFGPALLTEYFRKL

3D structure

• PDB: 5nsm Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation inTrypanosoma brucei.
• Chain: D
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K301 R375
• Catalytic site (residue number reindexed from 1): K299 R373

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BCT	D	A372 G374 R375 L402	A370 G372 R373 L400
BS02	MN	D	D294 D371 E373	D292 D369 E371
BS03	MN	D	K289 D294 D312 E373	K287 D292 D310 E371

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0005515 protein binding
• GO:0008233 peptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5nsm, PDBe:5nsm, PDBj:5nsm
• PDBsum: 5nsm
• PubMed: 28815215
• UniProt: Q385B0