Structure of PDB 5jji Chain D

Receptor sequence

>5jjiD (length=406) Species: 83334 (Escherichia coli O157:H7)

MNLTELKNTPVSELITLGENMGRKQDIIFAILKQHAKSGEDIFGDGVLEI
LQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPERY
FALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLID
ERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDV
IILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRFFGAARNVEEG
GSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDY
NRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTND
DFFEMM

3D structure

• PDB: 5jji Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor.
• Chain: D
• Resolution: 2.601 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	V284 L285 G287	V274 L275 G277
BS02	ADP	D	R366 K367	R356 K357
BS03	ADP	D	K181 G183 K184 T185 M186 F355	K171 G173 K174 T175 M176 F345
BS04	BEF	D	P180 K184 R212	P170 K174 R202

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008186 ATP-dependent activity, acting on RNA
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006353 DNA-templated transcription termination

Cellular Component

• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:5jji, PDBe:5jji, PDBj:5jji
• PDBsum: 5jji
• PubMed: 27856760
• UniProt: P0AG30|RHO_ECOLI Transcription termination factor Rho (Gene Name=rho)