Structure of PDB 5j32 Chain D

Receptor sequence
>5j32D (length=360) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
FGKKRYTITLLPGDGIGPEVVSIAKNVLQQAGSLEGVEFNFREMPIGGAA
LDLVGVPLPEETISAAKESDAVLLGAIGGYKWDNNEKHLRPEKGLLQIRA
ALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGIYFGEPRG
IKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLE
ASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFG
DILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLA
TILSAAMLLKYGLGEEKAAKRIEDAVLVALNNGFRTGDIYSAGTKLVGCK
EMGEEVLKSV
3D structure
PDB5j32 Structure and Mechanism of Isopropylmalate Dehydrogenase from Arabidopsis thaliana: INSIGHTS ON LEUCINE AND ALIPHATIC GLUCOSINOLATE BIOSYNTHESIS.
ChainD
Resolution1.933 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L48 Y181 K232 D264 D288 D292
Catalytic site (residue number reindexed from 1) L11 Y144 K195 D227 D251 D255
Enzyme Commision number 1.1.1.85: 3-isopropylmalate dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG D D288 D292 D251 D255
BS02 IPM D R136 R146 R174 Y181 D288 R99 R109 R137 Y144 D251
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003862 3-isopropylmalate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0051287 NAD binding
GO:0070403 NAD+ binding
Biological Process
GO:0009098 L-leucine biosynthetic process
GO:0009553 embryo sac development
GO:0009555 pollen development
Cellular Component
GO:0005829 cytosol
GO:0009507 chloroplast
GO:0009536 plastid
GO:0009570 chloroplast stroma
GO:0009941 chloroplast envelope

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Molecular Function
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Cellular Component
External links
PDB RCSB:5j32, PDBe:5j32, PDBj:5j32
PDBsum5j32
PubMed27137927
UniProtP93832|LEU32_ARATH 3-isopropylmalate dehydrogenase 2, chloroplastic (Gene Name=IMDH2)

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