Structure of PDB 5hm8 Chain D

Receptor sequence

>5hm8D (length=493) Species: 353152 (Cryptosporidium parvum Iowa II)

MESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGSL
HLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAWK
NETIEDYWVCLNDAMTWRNPNDKDKICGPNLIVDDGGDATLILHEGVKAE
IEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGML
KDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQ
SLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPI
CALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIA
NIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLV
NLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKE
LDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5hm8 2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.
• Chain: D
• Resolution: 2.85 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H51 S74 S79 D135 E209 N234 K239 D243 N244 C248 H354 H408 S416 Q420
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	D	K489 Y493	K487 Y491
BS02	ADN	D	H53 T55 E57 D137 E211 T212 K241 D245 H356 H410 M415	H51 T53 E55 D135 E209 T210 K239 D243 H354 H408 M413
BS03	NAD	D	T212 T213 T214 N246 G277 E278 V279 E298 I299 T331 N333 I354 G355 H356 N403	T210 T211 T212 N244 G275 E276 V277 E296 I297 T329 N331 I352 G353 H354 N401

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5hm8, PDBe:5hm8, PDBj:5hm8
• PDBsum: 5hm8
• UniProt: Q5CPH1