Structure of PDB 5hie Chain D

Receptor sequence
>5hieD (length=253) Species: 9606 (Homo sapiens) [Search protein sequence]
DWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLTPQQLQAFKNEVG
VLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKL
IDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLSGSILW
MAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMV
GRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLAR
SLP
3D structure
PDB5hie Activation Mechanism of Oncogenic Deletion Mutations in BRAF, EGFR, and HER2.
ChainD
Resolution3.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D576 K578 N580 N581 D594 S616
Catalytic site (residue number reindexed from 1) D123 K125 N127 N128 D141 S147
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 P06 D S465 V471 A481 K483 L514 I527 T529 W531 C532 F583 D594 F595 S17 V23 A33 K35 L61 I74 T76 W78 C79 F130 D141 F142 BindingDB: IC50=1.000000nM,EC50=529nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:5hie, PDBe:5hie, PDBj:5hie
PDBsum5hie
PubMed26996308
UniProtP15056|BRAF_HUMAN Serine/threonine-protein kinase B-raf (Gene Name=BRAF)

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