Structure of PDB 5a8w Chain D

Receptor sequence

>5a8wD (length=549) Species: 145261 (Methanothermobacter wolfeii)

EKKLFLKALKKKFEGEDPEEKSTNFYCFGGWEQSERKREFTEYAKKAAEK
RGGIPFYNPDIGVPLGQRKLMAYRVSGTDAYVEGDDLHFVNNAAIQQMVD
DIKRTVIVGMDTAHAVLEKRLGVEVTPETINEYMEVINHALPGGAVVQEH
MVEVHPGIVEDCYAKVFTGDDNLADELDKRILIDINKEFPEEQAEQLKSY
IGNRTYQVNRVPTIVVRACDGGTVSRWSAMQIGMSFISAYKLCAGEAAIA
DFSFAAKHADVIEMGTIMPARRARGPNEPGGVAFGTFADIVQASRVSDDP
ANVSLEVIAGAAALYDQVWLGSYMSGGVGFTQYATAAYTDDILDDFLYYG
MEYVEDKFGICGSEPTMDVVRDISTEVTLYSLEQYEEYPTLLEDHFGGSQ
RAAVAAAAAGCSTAFATGNSNAGVNGWYLSQILHKEAHSRLGFYGYDLQD
QCGASNSLSIRSDEGLIHELRGPNYPNYAMNVGHQPEYAGIAQAPHAARG
DAFCTNPLIKVAFADKDLSFDFTSPRKSIAKGALREFIPEGERDLIIPA

3D structure

• PDB: 5a8w Didehydroaspartate Modification in Methyl-Coenzyme M Reductase Catalyzing Methane Formation.
• Chain: D
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q151 Y336 G448
• Catalytic site (residue number reindexed from 1): Q148 Y333 G445
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TP7	D	R229 K260 H261	R226 K257 H258
BS02	TP7	D	R274 M327 F333 F446 M483 N484	R271 M324 F330 F443 M480 N481
BS03	F43	D	G330 V331 G332 F333 T334 Q335 Y336 G445 F446	G327 V328 G329 F330 T331 Q332 Y333 G442 F443
BS04	COM	D	Y336 F446 Y447	Y333 F443 Y444
BS05	F43	D	A148 V149 V150 Q151 M154 Q234 M237 A247	A145 V146 V147 Q148 M151 Q231 M234 A244

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

External links

• PDB: RCSB:5a8w, PDBe:5a8w, PDBj:5a8w
• PDBsum: 5a8w
• PubMed: 27467699
• UniProt: H7CHY2