Structure of PDB 4zh4 Chain D

Receptor sequence
>4zh4D (length=1151) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLF
CARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIEL
ASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQ
QILTEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNE
TNSETKRKKLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDG
GRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALL
DNGRRGRAITGSNKRPLKSLADKRVDYSGRSVITVGPYLRLHQCGLPKKM
ALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHPVL
LNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPL
TLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKG
EGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAKTSLKDT
TVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFAD
QIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGL
VTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSI
YMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQY
FISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMM
TPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADILVPRNTLLHEQWCDLL
EENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQS
IGEPGTQLTTGGLPRVADLFEARRPKEPAILAEISGIVSFGKETKGKRRL
VITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDGPEAPHDILRLRG
VHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFL
EGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFISAASF
QETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAA
G
3D structure
PDB4zh4 Structural Basis of Transcription Inhibition by CBR Hydroxamidines and CBR Pyrazoles.
ChainD
Resolution3.993 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 4OE D P750 I755 F773 P728 I733 F751 PDBbind-CN: -logKd/Ki=4.22,IC50=60uM
BS02 MG D D460 D462 D464 D438 D440 D442
BS03 ZN D C72 C85 C65 C78
BS04 ZN D C888 C898 C866 C876
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4zh4, PDBe:4zh4, PDBj:4zh4
PDBsum4zh4
PubMed26190576
UniProtP0A8T7|RPOC_ECOLI DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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