Structure of PDB 4tm4 Chain D

Receptor sequence

>4tm4D (length=414) Species: 345341 (Kutzneria sp. 744)

PTHDVVGVGFGPANLSLAVALEESPAALTSAFFERRASISWHQGMLLPAA
KMQVSFLKDLATFRNPASRFSFVSFLHERGRLVRFANNHDFFPTRREFHD
YLEWAESKLAHEVSYDSEVTAIRPGPGRPVDSVLVDVSTPEATRTVEARN
IVISTGLVPRMPAGVQSDEFVWHSSRFLDHFRDRDPRSLRRVAVAGGGQS
AAEIVRFLHDNRPDTVVHAIMPSYGYVVADNTPFANQIFDPAAVDDYFDG
SKQAKDAFWRYHRNTNYSVVDDEVIRDLYRRGYDDEVAGAPRLNFVNLAH
VVGAKRIADDTRVTVYSMAREESYDLDVDVLVCATGYDPMDPGDLLGELA
EHCVQDAEGRWQVDRDYRMVTTPDLRCGIYLQGGTEHTHGLSSSLLSNLA
TRSGEIVSSIERRK

3D structure

• PDB: 4tm4 Crystallographic Evidence of Drastic Conformational Changes in the Active Site of a Flavin-Dependent N-Hydroxylase.
• Chain: D
• Resolution: 2.632 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.59: L-lysine N(6)-monooxygenase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FDA	D	G18 G20 P21 A22 E43 R44 W50 H51 M61 V128 T164 G165 Y346 S403 L404 L405	G9 G11 P12 A13 E34 R35 W41 H42 M52 V119 T155 G156 Y337 S394 L395 L396
BS02	NAP	D	K60 Q62 R104 G205 G206 Q208 S209 E212 P231 N275 S277 V310 T344 G345	K51 Q53 R95 G196 G197 Q199 S200 E203 P222 N266 S268 V301 T335 G336

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004497 monooxygenase activity
• GO:0047091 L-lysine 6-monooxygenase (NADPH) activity

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:4tm4, PDBe:4tm4, PDBj:4tm4
• PDBsum: 4tm4
• PubMed: 25184411
• UniProt: A8CF85