Structure of PDB 4mne Chain D

Receptor sequence
>4mneD (length=285) Species: 9606 (Homo sapiens) [Search protein sequence]
DDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIREL
QVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQI
LGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQL
IDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPP
PDAKELELMPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPA
ERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLN
3D structure
PDB4mne Structure of the BRAF-MEK Complex Reveals a Kinase Activity Independent Role for BRAF in MAPK Signaling.
ChainD
Resolution2.8483 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D190 K192 S194 N195 D208 D217 T226
Catalytic site (residue number reindexed from 1) D125 K127 S129 N130 D143 D152 T161
Enzyme Commision number 2.7.12.2: mitogen-activated protein kinase kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ACP D L74 G75 A76 G77 V82 A95 K97 M143 M146 S150 Q153 K192 S194 L197 L9 G10 A11 G12 V17 A30 K32 M78 M81 S85 Q88 K127 S129 L132
BS02 MG D N195 D208 N130 D143
BS03 573 D G80 K97 V127 I141 D208 F209 G210 V211 S212 L215 I216 M219 G15 K32 V62 I76 D143 F144 G145 V146 S147 L150 I151 M154 BindingDB: EC50=7.2nM,IC50=35nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:4mne, PDBe:4mne, PDBj:4mne
PDBsum4mne
PubMed25155755
UniProtQ02750|MP2K1_HUMAN Dual specificity mitogen-activated protein kinase kinase 1 (Gene Name=MAP2K1)

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