Structure of PDB 4mih Chain D

Receptor sequence

>4mihD (length=578)

MFLDTTPFRADEPYDVFIAGSGPIGATFAKLCVDANLRVCMVEIGAADSF
TSKPMKGQVPIPGYHKKNEIEYQKDIDRFVNVIKGALSTCSIPTSNNHIA
TLDPSVVSNSLDKPFISLGKNPAQNPFVNLGAEAVTRGVGGMSTAWTCAT
PEFFAPADFNAPHRERPKLSTDAAEDARIWKDLYAQAKEIIGTSTTEFDH
SIRHNLVLRKYNDIFQKENVIREFSPLPLACHRLTDPDYVEWHATDRILE
ELFTDPVKRGRFTLLTNHRCTKLVFKHYRPGEENEVDYALVEDLLPSVKK
IYARSYVVACGAVATAQVLANSHIPPERDATIPTPLMPMLGKYITEQPMT
FCQVVLDSSLMEVVRNPPWPGLDWWKEKVARHVEAFPNDPIPIPFRDPEP
QVTIKFTEEHPWHVQIHRDAFSYGAVAENMDTRVIVDYRFFGYTEPQEAN
ELVFQQHYRDAYDMPQPTFKFTMSQDDRARARRMMDDMCNIALKIGGYLP
GSEPQFMTPGLALHLAGTTRCGLDTQKTVGNTHCKVHNFNNLYVGGNGVI
ETGFAANPTLTSICYAIRASNDIIAKFG

3D structure

• PDB: 4mih Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.
• Chain: D
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.10: pyranose oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	D	G22 P23 I24 E43 I44 G151 G154 M155 W159 T160 A162 H281 C283 C332 G333 A336 N586 N596 P597 T598	G22 P23 I24 E43 I44 G138 G141 M142 W146 T147 A149 H268 C270 C310 G311 A314 N547 N557 P558 T559
BS02	G3F	D	T160 Q454 D458 Y462 A551 H553 N596	T147 Q415 D419 Y423 A512 H514 N557

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0050233 pyranose oxidase activity
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:4mih, PDBe:4mih, PDBj:4mih
• PDBsum: 4mih
• PubMed: 24282677
• UniProt: Q6QWR1|P2OX_PHACH Pyranose 2-oxidase (Gene Name=p2ox)