Structure of PDB 4mfe Chain D

Receptor sequence

>4mfeD (length=594) Species: 347834 (Rhizobium etli CFN 42)

DRATKLLTYLADVTVNGHPEAKDRPKPLENAARPVVPYANGVKDGTKQLL
DTLGPKKFGEWMRNEKRVLLTDTTMRDGHQSLLATRMRTYDIARIAGTYS
HALPNLLSLECWGGATFDVSMRFLTEDPWERLALIREGAPNLLLQMLLRG
ANGVGYTNYPDNVVKYFVRQAAKGGIDLFRVFDCLNWVENMRVSMDAIAE
ENKLCEAAICYTGDILNSARPKYDLKYYTNLAVELEKAGAHIIAVKDMAG
LLKPAAAKVLFKALREATGLPIHFHTHDTSGIAAATVLAAVEAGVDAVDA
AMDALSGNTSQPCLGSIVEALSGSERDPGLDPAWIRRISFYWEAVRNQYA
AFESDLKGPASEVYLHEMPGGQFTNLKEQARSLGLETRWHQVAQAYADAN
QMFGDIVKVTPSSKVVGDMALMMVSQDLTVADVVSPDREVSFPESVVSML
KGDLGQPPSGWPEALQKKALGEKPYTVRPGSLLKEADLDAERKVIEKKLE
REVSDFEFASYLMYPKVFTDFALASDTYGPVSVLPTPAYFYGLADGEELF
ADIEKGKTLVIVNQAVSATDSQGMVTVFFELNGQPRRIKVPDRA

3D structure

• PDB: 4mfe Insights into the carboxyltransferase reaction of pyruvate carboxylase from the structures of bound product and intermediate analogs.
• Chain: D
• Resolution: 2.61 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D549 D655 K718 H747 H749 T882
• Catalytic site (residue number reindexed from 1): D77 D183 K246 H275 H277 T410
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	D	D549 K718 H747 H749	D77 K246 H275 H277
BS02	3PY	D	Q552 L619 R621 K718 T882	Q80 L147 R149 K246 T410
BS03	BTN	D	Y479 D482 R1066	Y9 D12 R593
BS04	MG	D	M534 R535 E537 D768	M62 R63 E65 D296

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:4mfe, PDBe:4mfe, PDBj:4mfe
• PDBsum: 4mfe
• PubMed: 24157795
• UniProt: Q2K340