Structure of PDB 4ima Chain D

Receptor sequence
>4imaD (length=460) Species: 9606 (Homo sapiens) [Search protein sequence]
QLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIG
PASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFSPLS
YRPVAIALDTKGPEIREVELVDYPNIIYIDDGLISLVENGGVGVNLPGPG
LSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISK
IENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLA
GKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGNF
PVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAA
FKCMAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVF
PLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSG
YTNIMRVLSI
3D structure
PDB4ima Energetic Coupling between an Oxidizable Cysteine and the Phosphorylatable N-Terminus of Human Liver Pyruvate Kinase.
ChainD
Resolution1.954 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R85 R132 K282 T340
Catalytic site (residue number reindexed from 1) R71 R116 K200 T258
Enzyme Commision number 2.7.1.40: pyruvate kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MN D E284 D308 E202 D226
BS02 FBP D L443 T444 T445 T446 S449 R501 G526 R528 P529 G530 S531 G532 Y533 T534 L361 T362 T363 T364 S367 R419 G444 R446 P447 G448 S449 G450 Y451 T452
BS03 ADN D E98 N102 E84 N88
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004743 pyruvate kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0030955 potassium ion binding
GO:0046872 metal ion binding
GO:0048029 monosaccharide binding
Biological Process
GO:0001666 response to hypoxia
GO:0006096 glycolytic process
GO:0007584 response to nutrient
GO:0009749 response to glucose
GO:0010038 response to metal ion
GO:0016310 phosphorylation
GO:0032869 cellular response to insulin stimulus
GO:0033198 response to ATP
GO:0042866 pyruvate biosynthetic process
GO:0051591 response to cAMP
GO:0071872 cellular response to epinephrine stimulus
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4ima, PDBe:4ima, PDBj:4ima
PDBsum4ima
PubMed23270483
UniProtP30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)

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