Structure of PDB 4hg3 Chain D

Receptor sequence
>4hg3D (length=292) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
KLKRVAVAQLCSSADLTKNLKVVKELISEAIQKKADVVFLPEASDYLSQN
PLHSRYLAQKSPKFIRQLQSSITDLVRDNSRNIDVSIGVHLPPSEQDLLE
GNDRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAI
PDIIESPLGKLGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIKTGEAH
WELLGRARAVDTQCYVLMPGQVGMHDLSDPEWEKRRESWGHSMVIDPWGK
IIAHADPSTVGPQLILADLDRELLQEIRNKMPLWNQRRDDLF
3D structure
PDB4hg3 Structures of enzyme-intermediate complexes of yeast Nit2: insights into its catalytic mechanism and different substrate specificity compared with mammalian Nit2
ChainD
Resolution1.93 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) N110 K127 E143 C169 T196
Catalytic site (residue number reindexed from 1) N107 K124 E140 C166 T193
Enzyme Commision number 3.5.1.128: deaminated glutathione amidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 AKG D K127 F131 C169 Y170 R173 F195 T199 K124 F128 C166 Y167 R170 F192 T196
Gene Ontology
Molecular Function
GO:0016787 hydrolase activity
GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
GO:0050406 [acetyl-CoA carboxylase]-phosphatase activity
GO:0110050 deaminated glutathione amidase activity
Biological Process
GO:0043605 amide catabolic process
Cellular Component
GO:0005575 cellular_component
GO:0005737 cytoplasm
GO:0005739 mitochondrion

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4hg3, PDBe:4hg3, PDBj:4hg3
PDBsum4hg3
PubMed23897470
UniProtP47016|NIT2_YEAST Deaminated glutathione amidase (Gene Name=NIT2)

[Back to BioLiP]